User: jonathanpa12

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Posts by jonathanpa12

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LncRNA and Immune-related genes
... Hello everyone I got a list of up-regulated and down-regulated LncRNAs and Immune-related genes from a differential analysis of a RNAseq. I would like to know whether any of the LncRNAs participate in the regulation of expression or function of those immune-related genes, but I don´t know if it´s p ...
tools rna-seq written 8 weeks ago by jonathanpa120 • updated 8 weeks ago by Antonio R. Franco4.5k
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Comment: C: filter before differential expression analysis
... Thank you so much for your help ...
written 9 weeks ago by jonathanpa120
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Comment: C: filter before differential expression analysis
... Exactly. And I would like to validate experimentally this analysis later, so I think if I can get more genes would be better. ...
written 9 weeks ago by jonathanpa120
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Comment: C: filter before differential expression analysis
... Sorry, I must learn to ask better. I used Deseq2 to make the differential expression analysis and I got 11 genes using as significance thresholds: Adjusted p-value: 0.05 and Log2 fold change: 1. Only one of the 11 genes is a immne-related gene of the list that I created manually. ...
written 9 weeks ago by jonathanpa120
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Comment: C: filter before differential expression analysis
... The final list of differentially of expressed gene shows only two immune-related gene. I was thinking that those genes usually have very low expression rates but biologically minimal differences in gene expression sometimes may produce significant changes in immune response, Because of this I though ...
written 9 weeks ago by jonathanpa120
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filter before differential expression analysis
... Hello, everyone I am analyzing a datasets of RNA-seq from different stages of cervical cancer. I would like to identify up or down-regulated immune related genes between the different stage. I have a list of the immune related genes and I would like to filter these genes before the differential exp ...
rna-seq written 9 weeks ago by jonathanpa120 • updated 9 weeks ago by i.sudbery7.8k

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