User: innyus

gravatar for innyus
innyus20
Reputation:
20
Status:
New User
Location:
Last seen:
1 month, 4 weeks ago
Joined:
5 months ago
Email:
i*****@utu.fi

Posts by innyus

<prev • 15 results • page 2 of 2 • next >
0
votes
0
answers
639
views
0
answers
Comment: C: Choosing the appropriate test for differential analysis: Limma or Mann-Whitney (
... Yes. Multiplex IHC which is then digitally quantified. ...
written 4 months ago by innyus20
2
votes
0
answers
639
views
0
answers
Forum: Choosing the appropriate test for differential analysis: Limma or Mann-Whitney (with BH correction)? Test for normality
... Hi everyone! Begginer at R. I´am currently analyzing immunohistochemistry expression in two different but related cancers. The data consists of 140 samples and over 250 different variables. I´ve done the Shapiro-Wilk test with Bonferroni correction to check for normality and only 11 out of 250 ce ...
R shapiro-wilk mann-whitney forum limma written 4 months ago by innyus20 • updated 4 months ago by heididunst10
5
votes
1
answer
186
views
6 follow
1
answer
Scale() function, is it always necessary?
... Hi all! I am a beginner in R. I am currently working on gene expression data (Nanostring). The data is normalized, house-keeping genes and low abundance have been removed and the data have been log2 transformed. I have done DE analysis with limma. Question: do I need to use scale(data) before r ...
gene scaling differential expression written 5 months ago by innyus20
0
votes
2
answers
159
views
2
answers
Comment: A: Differential gene expression
... Ok, thx! I think I was a little bit unclear in my question. I have a affy dataset (HGU plus 2.0) from GEO with 20 samples (10 for each cancer type). I use normalized data and I also changed the probes to gene IDs. I want to know is there a statistical pitfall of just pulling e.g. 100 genes from th ...
written 5 months ago by innyus20
0
votes
2
answers
159
views
2
answers
Differential gene expression
... Hi all, I am working with differential gene expression on an affy dataset (2 different cancers). I’ve done the analysis using the limma package with multiple test correction on all probes (over 50k). No problem there. My question: I want to use around 100 genes (for a certain pathway) to cluster ...
R limma differential gene expression written 5 months ago by innyus20

Latest awards to innyus

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1037 users visited in the last hour