User: l0o0

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l0o080
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China
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3 days, 7 hours ago
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4 years, 3 months ago
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Posts by l0o0

<prev • 41 results • page 1 of 5 • next >
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Comment: C: How to fix my broken GTF file?
... I think you can get gene region on genome by your gtf file. Ignore UTR region. You can try to get the start and end position of a gene. The start and end position should in the first and last exon's boundary, these exons should belong to the same gene. ...
written 3 days ago by l0o080
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Answer: A: Biopython on the web (Flask or Django)
... I think Flask is written in python, so you can handle this job as how you treat your data in python script. Here is a small flask app to upload a fasta file and then get sequence id of fasta file. import os from flask import Flask, request, redirect, url_for, send_from_directory fr ...
written 3 days ago by l0o080
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qPCR to validate gene FPKM is 0 in RNA-Seq
... Hi, I got my RNA-Seq expression Data in FPKM, then I pick some interested genes to validate by qPCR. The expression table of FPKM and qPCR in like below: [You can see this table](https://ibb.co/cHoOkk) or download from [google driver](https://drive.google.com/open?id=0Bxeiu-r4CJrJQkhBb3B2ekZvNkU ...
rna-seq qpcr written 3 days ago by l0o080
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Comment: C: what does miRNA* mean?
... Thank yourfor your answer. In my mind, novel miRNAs seem to be not in the miRbase but they have a miRNA-like structure. Is it necessary to check miRNA* for novel miRNA? ...
written 10 weeks ago by l0o080
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what does miRNA* mean?
... I am reading a paper about miRNA and confused by a fig. ![enter image description here][1] [1]: http://i.imgur.com/yKXw8Ja.png In "Novel miRNA for which miRNA* were found", what does miRNA* mean in the context? Does it mean reverse-complement sequence of miRNA? I hope someone could give me so ...
mirna written 10 weeks ago by l0o080 • updated 10 weeks ago by Michael Dondrup41k
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Comment: C: Combine multilple genome to one genome
... Yes, I will try to make a genome consensus sequence by alignments ;) ...
written 3 months ago by l0o080
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Comment: C: Combine multilple genome to one genome
... Now I have 5 genomes from different rice specie. If there would be a combination of genome, it will easy and fast to find some interesting region. It is just a lazy way. ...
written 3 months ago by l0o080
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Comment: C: Combine multilple genome to one genome
... Thank you for your advice, I will find some resources about genome graph representation. ...
written 3 months ago by l0o080
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Combine multilple genome to one genome
... I have a question about how to combine multiple genomes from different rice species to one genome. These rice species have close genetic relationship. I knon this question sounds weird, but I really want to find ways to combine these genomes. At first glance of this question, I will blast A geno ...
genome written 3 months ago by l0o080
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Comment: C: Error when running SNPEff
... The gff seems ok, I fixed this error by using version 4.1l. It's a strange error. ...
written 6 months ago by l0o080

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Popular Question 18 days ago, created a question with more than 1,000 views. For Volcano Plot Of Significantly Differential Exp Genes
Popular Question 4 weeks ago, created a question with more than 1,000 views. For Volcano Plot Of Significantly Differential Exp Genes
Popular Question 5 months ago, created a question with more than 1,000 views. For genes fpkm value of Coexpression module detected by WGCNA
Great Question 9 months ago, created a question with more than 5,000 views. For Volcano Plot Of Significantly Differential Exp Genes
Popular Question 12 months ago, created a question with more than 1,000 views. For How To Visualize Large Set Of Data(Fpkm Of Genes, 80 Data)
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Popular Question 20 months ago, created a question with more than 1,000 views. For How To Visualize Large Set Of Data(Fpkm Of Genes, 80 Data)
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Popular Question 2.8 years ago, created a question with more than 1,000 views. For Volcano Plot Of Significantly Differential Exp Genes

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