User: nmargu

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nmargu0
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Posts by nmargu

<prev • 4 results • page 1 of 1 • next >
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Comment: A: how to acces to htslib command
... Thank you so much! I am not able to code in C so I will try to find something more human-friendly for my purpose Thank you :) ...
written 5 months ago by nmargu0
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how to acces to htslib command
... Hi all! As I understood samtools use htslib package for other operations. I am interested in bam_cigar2qlen that for I can see in inside bam.c of the htslib files. I have installed htslib but I do not know how to use the htslib packages for my bam from samtools (or from whatever) I just do not know ...
alignment written 5 months ago by nmargu0
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Comment: C: TLEN value is negative for both paired reads
... Thanks so much or your answers, Now I understand why the sequence is the same! I am using Bowtie2 ...
written 5 months ago by nmargu0
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TLEN value is negative for both paired reads
... Hello all, I am making alignment of pair-end reads after Illumina sequencing 2x150 but my DNA fragments are mostly shorter. I assume because of the flags that they are correctly mapped. After trimming and using bowtie-2 I have noticed that the majority of my pairs have the same TLEN negative value. ...
alignment written 5 months ago by nmargu0 • updated 14 days ago by Sam50

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