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Posts by afeizi
... RiboSeq does not measure the amount of rRNA bound RNA present, Ribo-seq measures the ribosome protected fragments (RPFs) of mRNA being translated in a sample. Therefore, reads from Ribos-seq experiment can either represent the real translation of the mRNA or only can be the result of stochastic bind ...
... Still, I would argue seeing Ribo-seq reads mapped to the genes without RNA-seq readouts doesn't only mean you have a higher efficiency of translation for some very low abundant genes in mRNA level. It more means you maybe have some technical issues with RNA-seq design and it might be that you need t ...
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