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Can I still detect ambient RNA if I do not have filtered gene-barcode matrix?
ambient RNA SoupX scRNA-seq single-cell
updated 3.3 years ago by 8.9k • written 3.3 years ago by ▴ 60
3
votes
1
reply
1.3k
views
How to QC single-cell RNAseq data for tumor samples if data is right skewed?
scRNA QC distribution single-cell
3.4 years ago by SupBioInformatics ▴ 60
1
vote
2
replies
3.9k
views
How to use "SingleR" on the marker genes from `FindAllMarkers` for each cluster?
SingleR seurat scRNAseq scRNA
updated 2.6 years ago by ▴ 10 • written 3.4 years ago by ▴ 60
2
votes
1
reply
5.2k
views
Should I normalize individually or after merging all the data?
scRNA normalization seurat scale qc
3.4 years ago by SupBioInformatics ▴ 60
6
votes
6
replies
3.6k
views
What is the best practice of scRNA workflow for multiple patients and samples using Seurat
scRNA sing-cell RNA seurat integrate
updated 6 months ago by • 0 • written 3.4 years ago by ▴ 60
0
votes
1
reply
1.6k
views
How to convert metabolite name/KEGG compound ID to gene ID?
geneID KEGG metabolite
updated 3.5 years ago by 20 • written 3.6 years ago by ▴ 60
0
votes
0
replies
794
views
What are advantages to use globaltest, ORA and GSVA algorithms using different enrichment methods
RNA-Seq enrichment algorithm
3.6 years ago by SupBioInformatics ▴ 60
2
votes
2
replies
992
views
How to calculate foldchange for log2'd expression data?
fold-change t-test
updated 7 months ago by 43k • written 3.6 years ago by ▴ 60
13
votes
10
replies
5.2k
views
6 follow
What is difference between bulk RNAseq and mRNAseq?
RNA-Seq bulk
updated 3.6 years ago by 15k • written 3.6 years ago by ▴ 60
1
vote
2
replies
854
views
What is REL_PATHWAY from KEGGREST
keggrest KEGG pathway R
3.7 years ago by SupBioInformatics ▴ 60
1
vote
1
reply
1.9k
views
How to get KEGG pathway set with its corresponding metabolite ID/names?
metabolomics pathway KEGG signature GSEA
3.7 years ago by SupBioInformatics ▴ 60
1
vote
2
replies
1.2k
views
Should I do normalization to gene/features or samples?
normalization preprocessing RNA-Seq metabolomics
updated 3.7 years ago by ▴ 230 • written 3.7 years ago by ▴ 60
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