User: andresllucena
andresllucena • 0
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Posts by andresllucena
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... Hi, I downloaded microRNA expression data from TCGA (Isoform Expression Quantification files) using the TCGAbiolinks package from Bioconductor. Now I made a MDS plot using limma package function `plotMDS` but I have a weird distance between samples of same group (normal tissue group) and called it o ...
written 5 weeks ago by
andresllucena • 0
• updated
5 weeks ago by
seidel ♦ 7.4k
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... I'm sorry, I will correct that. I used values from samples after the first one trying to improve the example.
Thank you so much.
P.S.: function `pivot_wider` seem to be from tidyr package and not dplyr. ...
written 6 weeks ago by
andresllucena • 0
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... I have a data frame as follows:
miRNA_region barcode read_count
MIMAT0000062 TCGA-05-4244-01A-01T-1108-13 14492
MIMAT0000063 TCGA-05-4244-01A-01T-1108-13 8767
MIMAT0000064 TCGA-05-4244-01A-01T-1108-13 610
MIMAT0000065 TCGA-05-4244-01A ...
written 6 weeks ago by
andresllucena • 0
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... There is how i solve that:
The 3p and 5p strands corresponds to these MIMAT IDs miRNA_region column, then you'll have to sum counts with this same ID for each sample and then you'll have the raw counts that you need for differential expression analysis and so on.
First, delete rows of ''precursor' ...
written 6 weeks ago by
andresllucena • 0
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... yes it does http://ramuigib.blogspot.com/ ...
written 5 months ago by
andresllucena • 0
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