User: mina95921m

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mina95921m0
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Posts by mina95921m

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Comment: C: Why there are no reads for gene quantification but many reads can be quantified
... I use `-t gene` to count reads covering the full length of the gene which both include intron and exon region. so exon+intron =gene, one feature ,I don't know if my understanding is correct? ...
written 1 day ago by mina95921m0
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Comment: C: Why there are no reads for gene quantification but many reads can be quantified
... I'm sorry I didn't describe it clearly. Genequant=exon+intron region , exonquant means all exon region of the gene, so the result of exon.quant also one line per gene including all the exon region. Actually, I want to get reads abundance of intron region using genequant-exonquant. using IGV to che ...
written 1 day ago by mina95921m0
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Comment: C: Why there are no reads for gene quantification but many reads can be quantified
... only part of them, and the gene for example: >grep ENSMUSG00000058207 gene.quant ENSMUSG00000058207 chr12 104338486 104346144 + 7659 0 >grep ENSMUSG00000058207 exon.quant ENSMUSG00000058207 12;12;12;12;12 104338486;104340494;104342939;104344127;104345233 104338545;104341150;104 ...
written 2 days ago by mina95921m0
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Why there are no reads for gene quantification but many reads can be quantified for exon using featureCounts
... Hi, I used featureCounts to quantitate whole genebody and exon-only region of the same gene using the same bam files, and unexpectedly found that exon-only region successfully assigned 4000k fragments while 0 fragments assigned whole genebody region, so I wonder why did this happen and how to qu ...
exon quantification rna-seq written 2 days ago by mina95921m0

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