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Posts by katywithawhy11
... The ends of the reads are where Tn5 bound chromatin and inserted sequencing adapters, giving rise to the observed sequences. Thus, sequencing reads flank accessible chromatin. Tn5 binds 9 bp of DNA. That’s why we adjust the coordinates +4 for positive strand reads, and -5 for negative strand reads. ...
... Hi new user here although I've been lurking trying to find answers to my issue! I'm relatively new to bioinformatics but have been working on analyzing data from an ATAC-Seq experiments in Arabidopsis. I'm having issues with my data not piling up around TSS like ATAC-Seq data should.... I've trie ...
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