User: chritinedra

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Posts by chritinedra

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Comment: C: cell ranger agrr
... so i use the output from cell ranger count SSA.data <- Read10X(data.dir = "F:/tutorial seurat/cell count aggr/AGGSSA/outs/filtered_feature_bc_matrix") SSA <- CreateSeuratObject(counts = SSA.data, project = "SSA", min.cells = 3) dim(SSA) ... SSA <- RunUMAP(SSA, dims =1:15) DimPlot(SSA, r ...
written 16 days ago by chritinedra0
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Comment: C: cell ranger agrr
... exactly i would like to add this information in seurat , to tell him baracode with tail-1 are for exemple sample 1 and the other sample 2 , so i can after see which cluser belong to which sample but i cant find how to do it with seurat ...
written 16 days ago by chritinedra0
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Comment: C: cell ranger agrr
... the samples are B cells, first condition is a subpopulation of the second condition. so i have to campare them to see what distingue this subpopulation from the other b cells, if i use the seurat integration workflow, the clustering will be the same for both ...
written 16 days ago by chritinedra0
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cell ranger agrr
... hello i have a question , i integrated two data ( 2 samples of B cells) with cell ranger aggr and with filtered matrix data from cell ranger aggr , i apply the seurat script but i dont know how to distingue between my two samples after clustering i can't use split.by because there is no row to u ...
R written 16 days ago by chritinedra0

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