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Posts by nageshprabhu.k
... Hello all, I have a question regarding duplication levels shown in MultiQC report. The raw data went through HTStream for preprocessing and MultiQC was used to check. In the report I saw that when I ran multiQC on 3 different lanes of the same sample it had about 40% duplication for all three lanes ...
... Yes, that is right. One way to do this would be to extract transcript Ids with respect to non haplotypes using a simple grep command on the annotation(GTF) file and then extract information belonging to those Ids from the ref fasta file and save them as a new fasta file. ...
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