User: grx0326

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grx03260
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Posts by grx0326

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Comment: A: deeptools bamcoverage and bamcompare tss plot
... I have the same problem as you do. In IGV the INPUT seems to be lower than the treatment group, but after bamcompare the plot show negative values. Do you have any clues now? ...
written 5 weeks ago by grx03260
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Comment: C: What is the best peakcaller for H3 antibody?
... Make sense! I will think about it. Thank you ...
written 5 weeks ago by grx03260
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Comment: C: What is the best peakcaller for H3 antibody?
... I used DNase to cut the fixed chromatin and precipitate nucleosome with H3 antibody instead of the traditional ultrasonic way, sorry if I said “chip-seq”mislead you. I don`t see any fundamentally difference between this and an atac-seq because they cut and gathered almost the same area in genome--H3 ...
written 5 weeks ago by grx03260
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Comment: C: What is the best peakcaller for H3 antibody?
... Western blot is exactly what I did, but I am not sure if WB is enough. Just thinking chip-seq might be more accurate? ...
written 5 weeks ago by grx03260
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Comment: C: What is the best peakcaller for H3 antibody?
... I have a control sample ,just the same as normal chip-seq. Actually I am not sure if there`s a difference between my chip-seq and an ATAC-Seq, cause they both seem to have no specific preference to chromatin. If ATAC-Seq can have peaks in macs2, I guess my H3 experiment can also have peaks ...
written 5 weeks ago by grx03260
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Comment: C: What is the best peakcaller for H3 antibody?
... I am expecting a global decrease of H3 in a very specific mutant. Actually I`m not sure how to demonstrate this,chip-seq is just a naive approach ...
written 5 weeks ago by grx03260
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What is the best peakcaller for H3 antibody?
... Hello there! I am working on a chip-seq data of general H3 histone antibody. As expected, there is not much difference between treatment group and control group, and I got so few peaks using MACS2 that I can hardly proceed further annotation and GO analysis. I know it`s kind of weird to use such ...
chip-seq written 9 weeks ago by grx03260 • updated 9 weeks ago by hyperghjhon0

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