User: edoardoscali.93

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Posts by edoardoscali.93

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Blastn max target sequences
... Quick question, probably already asked (sorry in this case). How can i set "Max target sequences" in NCBI BLASTn equal to 1? The minimum value I can set from the website is 10... Thank you all for the help and for the attention. ...
ncbi blastn written 2 days ago by edoardoscali.930 • updated 2 days ago by agata88810
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Comment: C: Help on local alignment. Is there any way to automate the process?
... Ok, I will try to connect with the link now. ...
written 27 days ago by edoardoscali.930
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Comment: A: Help on local alignment. Is there any way to automate the process?
... Skype is better for me. I can join you after 18:00 (UTC+2) today. Otherwise tomorrow when you prefer. Edoardo ...
written 27 days ago by edoardoscali.930
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Comment: C: Help on local alignment. Is there any way to automate the process?
... Thanks Dunios, I need to enter the percentage of similarity level between OTUs sequences and the assigned species from the blast alignment. I will be very happy to receive direct assistance via video call. Let me know how we can manage it and thanks for the support! ...
written 27 days ago by edoardoscali.930
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Comment: C: Help on local alignment. Is there any way to automate the process?
... Thanks for your detailed answer, I will try to provide you more details. I state that I am still a master student (in forestry and environmental science) and that this type of work is new to me. So I apologize in advance and thank you for your patience and your invaluable help. From September to D ...
written 27 days ago by edoardoscali.930
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Help on local alignment. Is there any way to automate the process?
... I have a excel file with more than 10.000 rows from a meta-barcoding study on airborne fungi. Each rows correspond to an OTU and I would like to ask if is possible to include a separate column with information on similarity (%), like those from a local alignment in NCBI. I can do this operation m ...
ncbi otu next-gen metabarcoding alignment written 28 days ago by edoardoscali.930 • updated 7 days ago by Biostar ♦♦ 20
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Comment: A: Working in R with HTS cluster analysis data
... This is an example database of how my data is arranged: my_data <- tibble(tag = c("tag_1", "tag_2", "tag_3", "tag_4", "tag_5"), sampler = c ("sampler_1", "sampler_2", "sampler_1", "sampler_3", "sampler_2"), site = c("site_1", "site_2", "site_2", "site_2", "site_1"), ...
written 4 months ago by edoardoscali.930 • updated 4 months ago by GenoMax96k
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Working in R with HTS cluster analysis data
... Good morning, I'm working with a large data set. Is a table with cluster analysis data from an HTS sequencing. I have a problem with constant crushing of R studio, especially when I try to make plots and merging 2 data frame. Sometimes I have the crushing error (session aborted) also when I try to l ...
vegan R hts metabarcoding tidyverse written 4 months ago by edoardoscali.930

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