Latest
Open
RNA-Seq
ChIP-Seq
SNP
Assembly
Tutorials
Tools
Jobs
Forum
Planet
All »
Home
Log In

User: abhilashtripathi10

gravatar for abhilashtripathi10
Reputation:
0
Status:
New User
Last seen:
1 week ago
Joined:
4 months, 3 weeks ago
Email:
a*****************@gmail.com

Profile information, website and location are not shown for new users.

This helps us discourage the inappropriate use of our site.

Posts by abhilashtripathi10

<prev • 7 results • page 1 of 1 • next >
0
votes
0
answers
142
views
0
answers
Comment: C: Formatting BAM file and GTF file for feature counts
... Thank you for redirecting. ...
written 9 weeks ago by abhilashtripathi10 • 0
0
votes
0
answers
142
views
0
answers
Formatting BAM file and GTF file for feature counts
... I am trying to format the identifiers in my BAM file to match with the GTF file. I used Samtools: IdxStats tool on my BAM file and I got the following output: https://ibb.co/10rT9tL I am getting zero counts for RNA seq reads after using featurecount tool in galaxy. I suspect there is some problem w ...
alignment featurecounts rna-seq bam written 9 weeks ago by abhilashtripathi10 • 0
0
votes
0
answers
157
views
0
answers
Comment: C: Problem with recognizing adapters while using Cutadapt or Trimmomatic
... Thank you. I will take care of it next time. ...
written 11 weeks ago by abhilashtripathi10 • 0
0
votes
0
answers
157
views
0
answers
Problem with recognizing adapters while using Cutadapt or Trimmomatic
... I got my samples sequenced from a commercial facility. They used the following adapter sequences for sequencing Sample ID Index i7 Index i5 S0A GTTCTTCTGT CCAAGGTATT S0B CCAAGGTATT GTTCTTCTGT S0C TGTGGAGAGG AACGAACCGG S5A TTCCTTGAGG TGCTGT ...
rna-seq written 11 weeks ago by abhilashtripathi10 • 0 • updated 11 weeks ago by Ram ♦ 32k
0
votes
1
answer
372
views
1
answers
Comment: C: Read length and sequencing depth for RNA-seq (for differential gene expression a
... Thank you for the suggestion. I am trying to be on the low end with more biological replicates as you suggested. ...
written 4 months ago by abhilashtripathi10 • 0
0
votes
1
answer
372
views
1
answers
Comment: C: Read length and sequencing depth for RNA-seq (for differential gene expression a
... Thank you for your suggestion. yes, because of budget constraints I am trying to go for 10-20million reads (50bp, SE)/sample. I have three biological replicates for each sample. ...
written 4 months ago by abhilashtripathi10 • 0
1
vote
1
answer
372
views
1
answer
Read length and sequencing depth for RNA-seq (for differential gene expression analysis) of bacterial RNA
... Hello Everyone My group in my lab wants to elucidate differential gene expression during bacterial biofilm growth on some material surfaces. For the same, I want to know what should be the minimum read length, total no. reads, and whether I should go for PE or SE RNA sequencing. I went through the ...
gene next-gen rna-seq sequencing written 4 months ago by abhilashtripathi10 • 0

Latest awards to abhilashtripathi10

No awards yet. Soon to come :-)
Content
Help
Access
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 2290 users visited in the last hour
_