User: abhilashtripathi10
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Posts by abhilashtripathi10
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... Thank you for redirecting. ...
written 9 weeks ago by
abhilashtripathi10 • 0
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... I am trying to format the identifiers in my BAM file to match with the GTF file. I used Samtools: IdxStats tool on my BAM file and I got the following output: https://ibb.co/10rT9tL
I am getting zero counts for RNA seq reads after using featurecount tool in galaxy. I suspect there is some problem w ...
written 9 weeks ago by
abhilashtripathi10 • 0
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... Thank you. I will take care of it next time. ...
written 11 weeks ago by
abhilashtripathi10 • 0
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... I got my samples sequenced from a commercial facility. They used the following adapter sequences for sequencing
Sample ID Index i7 Index i5
S0A GTTCTTCTGT CCAAGGTATT
S0B CCAAGGTATT GTTCTTCTGT
S0C TGTGGAGAGG AACGAACCGG
S5A TTCCTTGAGG TGCTGT ...
written 11 weeks ago by
abhilashtripathi10 • 0
• updated
11 weeks ago by
Ram ♦ 32k
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... Thank you for the suggestion. I am trying to be on the low end with more biological replicates as you suggested. ...
written 4 months ago by
abhilashtripathi10 • 0
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... Thank you for your suggestion. yes, because of budget constraints I am trying to go for 10-20million reads (50bp, SE)/sample. I have three biological replicates for each sample. ...
written 4 months ago by
abhilashtripathi10 • 0
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... Hello Everyone
My group in my lab wants to elucidate differential gene expression during bacterial biofilm growth on some material surfaces. For the same, I want to know what should be the minimum read length, total no. reads, and whether I should go for PE or SE RNA sequencing. I went through the ...
written 4 months ago by
abhilashtripathi10 • 0
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