User: daniela.paola.s.p

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New User
Location:
Brazil/Montes Claros-MG/Universidade Estadual de Montes Claros
Last seen:
20 hours ago
Joined:
2 weeks, 3 days ago
Email:
d****************@gmail.com

Posts by daniela.paola.s.p

<prev • 23 results • page 1 of 3 • next >
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... I fixed the mistakes... now, when I run: ---------- >targets <- factor(targets$group) >design <- model.matrix(~0+targets) >colnames(design) <- levels(targets) >fit <- lmFit(project.bgcorrect.norm.filt.mean$E, design) >contrasts<-makeContrasts(habits-non_habits,levels ...
written 2 days ago by daniela.paola.s.p30
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... I think I did something wrong... > levels(targetinfo$groupnon_habits) NULL ...
written 2 days ago by daniela.paola.s.p30
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... My Variables already have: >habits >non_habits ...
written 2 days ago by daniela.paola.s.p30
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... Hey Kevin! When I try proceed with DEA analysis ,I'm having a litle troubles. Can you help-me ? design<- model.matrix(~ targetinfo$group) > fit <- lmFit(project.bgcorrect.norm.filt.mean$E, design) > contrasts<-makeContrasts(habits-non_habits,levels = design) Erro ...
written 3 days ago by daniela.paola.s.p30 • updated 2 days ago by Kevin Blighe68k
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... Ok, in this case, can I use "project.bgcorrect.norm.filt.mean$E" or I have to extract the mean in another way ? ...
written 3 days ago by daniela.paola.s.p30
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... Hi Kevin! How are you ? Using your responses, I did the pre process from raw data! (thank you very much for this!!) Now I'm going to proceed with differential expression analysis. Can I use the limma package for this tipe of data ? One more question, why i need summarise across genes b ...
written 3 days ago by daniela.paola.s.p30
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... Sim! Tento agora e te retorno! ...
written 11 days ago by daniela.paola.s.p30
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... Hey Kevin! Sorry for my late response! Finally i used the merge function , to align the probes with the expression matrix (as annot). When I try to merge the controls with the expression matrix , R provides me: Error in merge.data.frame(raw_int, controls, by.x = "Probe_Id", all.x = TRUE, : 'by ...
written 11 days ago by daniela.paola.s.p30
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... Hey Kevin! I found some articles and I'm watching some classes (videos) about. I hope return to talk with you soon :) ...
written 14 days ago by daniela.paola.s.p30
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Comment: C: GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
... There's no problem with this... I will read this article and trying to understand . Unfortunately I just have a few people help me( also trying understand ). Thanks for help me ...
written 14 days ago by daniela.paola.s.p30

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