User: intern

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intern0
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Posts by intern

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Comment: C: De novo transcriptom assembly and differential expression analysis.
... Ok thanks. I don't know if it is relevant also but I have 500,000,000 transcripts whereas reference transcriptome of closely related species only have 250,000, that's why I am trying to reduce my data. But I think after cd hit at 95 % and filtering according to mapping quality there are not a lot of ...
written 11 days ago by intern0
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Comment: A: De novo transcriptom assembly and differential expression analysis.
... Ok thanks for your advice. Also I would like to create a reference transcriptom for my target organism. So if I understand well I can filter low expression transcripts (according to TPM and size for instance) for this reference transcriptom and annote it. But for differential expression it is bet ...
written 15 days ago by intern0
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De novo transcriptom assembly and differential expression analysis.
... Hello all ! I am writing this post to expose my concerns regarding the methodology I am following. My goal is to perform de novo assembly (there is no reference transcriptome nor genome yet for my target organism) with 2 different conditions and 5 samples in each condition. And then to run function ...
gene assembly rna-seq written 17 days ago by intern0

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