User: Dr SKY

gravatar for Dr SKY
Dr SKY20
Reputation:
20
Status:
New User
Location:
Tezpur University, INDIA
Last seen:
8 hours ago
Joined:
2 months ago
Email:
a*****************@gmail.com

I am a PhD student in Systems Biology at Tezpur University, India. I am trying to figure out Biostatistics and programming knowledge all by myself. Any help form this site will bring great difference in my career.

Posts by Dr SKY

<prev • 9 results • page 1 of 1 • next >
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Comment: C: RNA Seq FAQ: What if the gene was never present/expressed in control sample?
... Thank you for your response. ...
written 25 days ago by Dr SKY20
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RNA Seq FAQ: What if the gene was never present/expressed in control sample?
... This is a very simple question and forgive me for sounding dumb in this case What if a gene was never expressed in the control sample? How do you determine the expression value change in treated condition using RNA Seq (if the gene expressed only under treated condition)? Will the gene be considere ...
rna-seq written 26 days ago by Dr SKY20 • updated 26 days ago by ATpoint46k
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Comment: C: Huge difference between P and adj P value
... Thank you so very much. I can now finally proceed to the next level. ...
written 26 days ago by Dr SKY20
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Comment: C: Huge difference between P and adj P value
... Thank you for your reply ...
written 26 days ago by Dr SKY20
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Huge difference between P and adj P value
... Hello friends, I recently analysed a RNA-Seq data and yielded DEGs using DESeq2. Everything was fine until I figured out that on applying P value 0.05, i am left with 1,700 DEGs whereas on considering and applying Padj 0.05, I am left with only 127 DEGs??? isn't this surprising ? How can there be s ...
rna-seq written 4 weeks ago by Dr SKY20 • updated 4 weeks ago by i.sudbery11k
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Answer: A: Importing KEGG pathways into cytoscape
... Hello, I found you finally. I am going through the same prob....haha If you have found the way out. Please let me know Thanks in advance However, you may use cytoKEGG plugin to import the same...This initially gives you the exact image like in KEGG but the prob that even i am facing is that it ...
written 5 weeks ago by Dr SKY20
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Not able to annotate KGML files in CYTOSCAPE
... I am trying to annotate my gene expression values into KEGG pathway diagrams for which i have taken the help of KEGG scape and cytoKEGG plugin of CYTOSCAPE. The problem that i am facing is that just after importing the KGML files which at first appears to resemble the image from KEGG in the cytoscap ...
rna-seq written 5 weeks ago by Dr SKY20 • updated 5 weeks ago by GenoMax96k
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Comment: C: RNA-SEQ on RICE
... Hello sir, Thank you for your response and kind concern. I have used the following tools and files Alignment : Hisat2 Counting : FeatureCounts reff genome : Os-Nipponbare-Reference-IRGSP-1.0 rice plant genome from RAP-DB gtf: Os-Nipponbare-Reference-IRGSP-1.0 rice plant annotation file (gtf) W ...
written 9 weeks ago by Dr SKY20
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RNA-SEQ on RICE
... Hello , I am a beginner in RNA-Seq analysis. I am particularly working on Rice transcriptome using RNA-Seq pipeline. I have done DESeq2 analysis and have yielded DEG's for rice. I have found the list containing types of IDs 1) Os id 2) ENSRNA___ 3) EPlORYSAT__ and 4) gene- XYZ . I am confused on ...
rice transcriptomics rna-seq written 9 weeks ago by Dr SKY20

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