User: rnaka09

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rnaka0930
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Posts by rnaka09

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Comment: C: Differential expression analysis with few genes. What could have happened?
... Thanks for the explanations and suggestions. I did the pairplot and I think there's not much else to do. ![pairplot][1] [1]: https://cdn1.bbcode0.com/uploads/2021/1/8/9767102d4cc8f8d0f96d4859197ee74b-full.png ...
written 18 days ago by rnaka0930
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Comment: C: Differential expression analysis with few genes. What could have happened?
... Has the image above shows, per group, look like a little mess...! ...
written 19 days ago by rnaka0930
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Comment: C: Differential expression analysis with few genes. What could have happened?
... Thanks for the suggestions! The edgeR code is: **1 - created Dgelist** dge_N_agua=DGEList(fc_Nel_agua, group = coldata_N_agua$Group) **2 - Normalize counts** dge_N_agua=calcNormFactors(dge_N_agua, method = 'TMM') **3 - Common and tagwise dispersion** dge_N_agua=estimateCommonDisp(d ...
written 19 days ago by rnaka0930 • updated 19 days ago by ATpoint44k
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Differential expression analysis with few genes. What could have happened?
... Hello everyone! In my PhD thesis, I am working with differential expression analysis, trying to verify genes related to thermoregulation in beef cattle. I have 66 sequenced, paired-end RNA samples, divided into 2 lanes per sample. The first steps were to check the quality of these samples by FASTQC ...
alignment rna-seq sequencing written 20 days ago by rnaka0930 • updated 20 days ago by swbarnes29.4k

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