User: kdcastillo

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Posts by kdcastillo

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Comment: A: Reads detected/"spill over" on introns after using bamCoverage function from dee
... ![enter image description here][1]Hello! I agree that it might be due to bin size, I tried the default and bin size of 10, and I am still getting the same issue. But I attached the script (for bin size 10) and the example image of the problem I'm seeing. bamCoverage -b filename.bam -o filename. ...
written 12 days ago by kdcastillo0 • updated 11 days ago by i.sudbery10k
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Reads detected/"spill over" on introns after using bamCoverage function from deepTools
... Hello! We recently used the recent version of bamCoverage from the deepTools suite for our ribo-seq and RNA-seq bam files to generate bigwig files. The conversions ran smoothly, however when we loaded the bigwig files into IGV, we noticed some reads spilling over to some exon-intron junctions when w ...
bamcoverage intron bigwig deeptools written 14 days ago by kdcastillo0 • updated 2 days ago by Biostar ♦♦ 20

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