User: dreamfeathers08

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d**************@gmail.com

Posts by dreamfeathers08

<prev • 16 results • page 1 of 2 • next >
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Comment: C: Getting 0 bytes or 1 line from galaxy
... Hi, Noted! Thank you! ...
written 4 days ago by dreamfeathers0810
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Comment: C: Getting 0 bytes or 1 line from galaxy
... Hi, I'll have a look at it. Thank you. ...
written 4 days ago by dreamfeathers0810
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Getting 0 bytes or 1 line from galaxy
... Hi, I have an existing dataset from nanopore run in fast5 format. I'm using GALAXY to convert it into fastq format. The tools which I've trialled from the tools list are : 1) extract fastq in tabular format from a set of fast5 files and 2) extract read in fasta or fastq format from nanopore files. ...
sequence written 5 days ago by dreamfeathers0810
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Comment: C: Need help on installing poretools
... Hi Pierre, Thank you for your explanation. It helps in my understanding. ...
written 5 days ago by dreamfeathers0810
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Need help on installing poretools
... Hi, I'm new to bioinformatics. I'm trying to install poretools. I've followed the steps from here: https://github.com/arq5x/poretools/blob/master/docs/content/installation.rst (basic installation) At the second step on updating the PATH...may I know what does it mean? how should I incorporate it ...
software error written 6 days ago by dreamfeathers0810
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Comment: C: Uploaded bam file into IGV but couldn't see anything
... Hi, Okay! Understood! Thank you for your help. Really appreciate it. ...
written 10 days ago by dreamfeathers0810
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Comment: C: Uploaded bam file into IGV but couldn't see anything
... Hi, Sorry for late reply. The forum doesn't allow me to post more than 5 comments in 6 hours. Back to your last suggestion: 1) Do you mean I should have 2 bamfiles after alignment? 2) my script should be like this?: minimap2 -ax map-ont -t 4 refseq.mmi -o input_nanofiltered.fastq.gz --secondary ...
written 10 days ago by dreamfeathers0810
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Comment: C: Uploaded bam file into IGV but couldn't see anything
... I'm confused, I only have one bamfile. All the samtools viewing done so far have been conducted on this one bamfile. I've trialed the script: samtools view output_file.bam | head -20 I got no outputs..... ...
written 11 days ago by dreamfeathers0810
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Comment: C: Uploaded bam file into IGV but couldn't see anything
... HI, Thank you for your reply. 1) What would be considered as an un-obfuscated file name? 2) Do you mean the bamfile which were not indexed? ...
written 11 days ago by dreamfeathers0810
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Comment: C: Uploaded bam file into IGV but couldn't see anything
... Hi, Ah...., no alignment. That's disappointing. Would you be able to help oversee the pathway that I've done...it needs experience to actually see where could potentially gone wrong.. 1) After getting the first 200 fastq files (each with 4000 reads = total would be 80000 reads), I combine them in ...
written 11 days ago by dreamfeathers0810

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