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116,028 results • Page
1 of 2321
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Votes
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0
votes
0
replies
8
views
Convert to ENTREZGENE
maize
ENTREZGENE
33 minutes ago by
sansan_96
▴ 50
0
votes
0
replies
14
views
Synthetic lethality and cancer database
interaction
lethal
synthetic
2 hours ago by
Shicheng Guo
★ 9.4k
0
votes
0
replies
23
views
Tissue specific mRNA expression dataset in human and mouse
tissue
specificity
3 hours ago by
Shicheng Guo
★ 9.4k
4
votes
9
replies
882
views
Error in openning FastQC
fastqc
igv
updated 4 hours ago by
GenoMax
140k • written 7 months ago by
huynhthienphai
• 0
0
votes
1
reply
67
views
vcf file: number of samples does not match number of columns
vcf
bcftools
updated 4 hours ago by
Pierre Lindenbaum
160k • written 5 hours ago by
ekirsch
• 0
0
votes
4
replies
149
views
Is it reasonable to discard reads that show variation of quality across its length?
Trimming
3 hours ago by
BRENO
• 0
0
votes
2
replies
99
views
Error running local blastn in R using system2
blastn
NCBI
R
updated 4 hours ago by
Mensur Dlakic
★ 26k • written 7 hours ago by
Harrison
• 0
793
votes
167
replies
139k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 9 weeks ago by
Biostar
2.6k • written 7.3 years ago by
Istvan Albert
100k
15
votes
9
replies
583
views
Forum:
F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 6 hours ago by
Dave Carlson
★ 1.7k • written 10 hours ago by
Ann
★ 2.4k
0
votes
0
replies
49
views
News:
Online course: Reproducibility in Bioinformatics
Singularity
Version-Control
Docker
Containers
updated 7 hours ago by
Ram
43k • written 7 hours ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
44
views
News:
Online course: Analysis of Prokaryotic Pangenomes (Last call)
Prokaryotic
Bacterial-Genomics
Pangenomics
updated 7 hours ago by
Ram
43k • written 8 hours ago by
carlopecoraro2
★ 2.4k
1
vote
9
replies
3.5k
views
Error in CIBERSORTx
RNA-seq
CIBERSORT
LM22
updated 8 hours ago by
Ram
43k • written 17 months ago by
Manav
• 0
0
votes
1
reply
112
views
Annotation of Complex genomes using NCBI Genome Workbench
Genome-Workbench
MAKER
updated 9 hours ago by
Lissa Cruz Saavedra
• 0 • written 22 hours ago by
SAsciences
• 0
0
votes
0
replies
53
views
Is RNAseq data from a monoclonal cell line technically considered scRNAseq?
scRNA
RNAseq
9 hours ago by
sithlordashley
• 0
0
votes
1
reply
61
views
Looking for RPIP Illumina kit probe sequences
Sequences
Illumina
updated 9 hours ago by
GenoMax
140k • written 10 hours ago by
David Tachiquín
• 0
0
votes
9
replies
264
views
Low number of both surviving reads after trimming
trimmomatic
trimming
4 hours ago by
Jay
• 0
0
votes
2
replies
113
views
ONT direct RNA sequencing
DRS
ONT
updated 8 hours ago by
Ram
43k • written 15 hours ago by
Karina
• 0
0
votes
1
reply
78
views
Number of CPUs to use by DRAP for transcriptomic assmbly
DRAP
CPU
transcriptomics
updated 9 hours ago by
GenoMax
140k • written 11 hours ago by
Mohamed Abderrahmane
▴ 10
2
votes
2
replies
87
views
Gene reads all zero for each sample
R
salmon
DE
DESeq2
11 hours ago by
DYLAN NICO
• 0
0
votes
2
replies
173
views
Can I have multiple active GDC download tokens?
TCGA
updated 11 hours ago by
Zhenyu Zhang
★ 1.1k • written 3 days ago by
mrk
• 0
0
votes
0
replies
55
views
Contradictions in GSEA results
GSEA
Enrichment-analysis
PEA
updated 11 hours ago by
Ram
43k • written 11 hours ago by
Tolga
▴ 20
0
votes
0
replies
50
views
Illumina Dna Prep kit for sequencing phage linear genomes
tagmentation
transposons
phagetermini
sequencing
12 hours ago by
alenew.am
• 0
0
votes
3
replies
297
views
FindAllMarkers not working (Error (data layers are not joined. Please run JoinLayersWarning: When testing 1 versus all)
FindAllMarkers
seurat
re-clustering
11 hours ago by
Nitin
• 0
0
votes
1
reply
85
views
(sc)RNA-seq dataset for these cell lines: HEK293, HeLa, PC3 and U2OS
scRNA-seq
updated 12 hours ago by
Ram
43k • written 12 hours ago by
chiditatawmbi
• 0
0
votes
1
reply
108
views
Normalization strategies for comparing mapped reads across samples in RNA-seq
normalization
rnaseq
updated 11 hours ago by
Ram
43k • written 13 hours ago by
Picasa
▴ 640
0
votes
0
replies
67
views
How to change alternate conformation occupancy ?
Bio.PDB.Atom
Bio.PDB
biopython
updated 11 hours ago by
Ram
43k • written 14 hours ago by
udakshi
• 0
0
votes
0
replies
85
views
Training
chip-seq
ScATAC-seq
14 hours ago by
Manhezz
• 0
1
vote
2
replies
127
views
Issue with making local BLAST database
R
mitochodrial
diatoms
blast
ncbi
updated 8 hours ago by
Ram
43k • written 14 hours ago by
Begonia_pavonina
▴ 140
1
vote
1
reply
278
views
diffbind : killed dba.count(DBsample, bParallel=FALSE)
diffbind
r
atacseq
15 hours ago by
Pierre Lindenbaum
160k
1
vote
2
replies
115
views
Flow Cytometry Data Analysis by Seurat
Seurat
updated 10 hours ago by
Ram
43k • written 15 hours ago by
gdfsnkfns
• 0
1
vote
11
replies
295
views
struggle to get fasta files from ucsc goldenPath
ucsc
getfasta
fasta
updated 9 hours ago by
GenoMax
140k • written 15 hours ago by
Lila M
★ 1.2k
2
votes
2
replies
112
views
Volcano Plot Output Inquiry: Graphs Facing Down
rstudio
volcano-plot
updated 11 hours ago by
dariober
14k • written 16 hours ago by
Pumla
• 0
0
votes
1
reply
87
views
Merging featureCounts output for different species
featureCounts
RNA-seq
updated 8 hours ago by
Ram
43k • written 17 hours ago by
Christian
• 0
2
votes
2
replies
131
views
Interproscan taking so much time
slurm
interproscan
transcriptomics
updated 16 hours ago by
dthorbur
★ 1.7k • written 17 hours ago by
Mohamed Abderrahmane
▴ 10
0
votes
0
replies
66
views
Combine rename and rmdup in SeqKit to remove duplicate sequences and append N in header? Sort by occurence?
seqkit
fasta
17 hours ago by
Broccoli
• 0
0
votes
0
replies
63
views
Is there a good tool/reference for PBP and porin analysis
PBP
porin
17 hours ago by
blur
▴ 280
0
votes
0
replies
73
views
meta-analysis on RNA seq AND microarray results
microarray
RNA
18 hours ago by
liuyibin2005
• 0
49
votes
30
replies
11k
views
13 follow
Tutorial:
How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
documentation
meta
how-to
updated 14 hours ago by
Istvan Albert
100k • written 8.1 years ago by
Ram
43k
0
votes
0
replies
59
views
SPOTlight deconvolution logFC, logFC cohen or mean.AUC
spatial-transcriptomics
SPOTlight
logFC
deconvolution
updated 12 hours ago by
Ram
43k • written 18 hours ago by
M'zelle
• 0
0
votes
5
replies
231
views
calculate mismatch rate from VCF file
mismatch-rate
VCF
updated 12 hours ago by
Ram
43k • written 1 day ago by
Dora
▴ 10
2
votes
4
replies
317
views
Hybrid assembly Pacbio- Nanopore
long-reads
hybric
assembly
genome
short-reads
9 hours ago by
Lissa Cruz Saavedra
• 0
0
votes
0
replies
78
views
In scRNA, How should I set QC standards?
scRNA
QC
21 hours ago by
mnx0723
• 0
0
votes
2
replies
241
views
Error occurs when migrate-n software performs "make mpis"
migrate-n
23 hours ago by
lingxue
• 0
0
votes
0
replies
78
views
How to check if a few pathways have relationship?
MSigdb
REACTOME
KEGG
1 day ago by
Chris
▴ 260
2
votes
10
replies
718
views
Only one read per run - Trying to use CellRangerv7
Fastq
CellRanger
updated 12 hours ago by
Ram
43k • written 5 weeks ago by
Sky
▴ 10
3
votes
2
replies
284
views
FDR and Bonferroni
FDR
Bonferroni
updated 1 day ago by
joe
▴ 470 • written 3 days ago by
song
• 0
0
votes
1
reply
488
views
Which fasta files are assembled transcripts IDBA_tran assembler ?
RNA-Seq
Assembly
updated 1 day ago by
weidonglu
• 0 • written 3.8 years ago by
sunnykevin97
▴ 980
0
votes
0
replies
93
views
doing some ontology enrichment analysis
GO
1 day ago by
aleksk779
• 0
2
votes
1
reply
189
views
Choosing an FC Threshold
statistics
rna-seq
updated 1 day ago by
Gordon Smyth
★ 6.9k • written 1 day ago by
Netanel
• 0
0
votes
1
reply
127
views
Converting AutoDock-Vina PDBQTs to PDB files
Docking
AutoDock
Vina
PDBQT
PDB
updated 1 day ago by
Mensur Dlakic
★ 26k • written 1 day ago by
Ricard
• 0
116,028 results • Page
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Recent Votes
Answer: log2(TPM+1)
Comment: Error in openning FastQC
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
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Comment: Is it reasonable to discard reads that show variation of quality across its leng
by
BRENO
• 0
Yikes. Well, this is from an Illumina MiniSeq. Sure, I will use alignment, but only to remove host reads. Then the remaining reads will go …
Comment: Is it reasonable to discard reads that show variation of quality across its leng
by
GenoMax
140k
This is going to be tough to diagnose. Are you going to align to a reference? If so go ahead with the alignments to see if alignments turn …
Comment: Is it reasonable to discard reads that show variation of quality across its leng
by
BRENO
• 0
Thank you for replying. I have separated the reads with at least one N on them and they account for 0.2% of the total reads in the file (8'…
Comment: Error running local blastn in R using system2
by
Mensur Dlakic
★ 26k
The `-db data/nt_euk` switch means that in your current directory you have a subdirectory called `data`, and all the `nt_euk` files are in …
Comment: Low number of both surviving reads after trimming
by
Jay
• 0
Thank you for your opinion. I will try with lower LEADING and TRAILING bases. By the way, I tried to set `keepBothReads:true` through sear…
Comment: Error in openning FastQC
by
GenoMax
140k
At this point it would be beneficial to learn some of the basics of command line. Please use this UNIX tutorial since you seem to be new to…
Comment: vcf file: number of samples does not match number of columns
by
Pierre Lindenbaum
160k
> ore or have any suggestions for fixing it? it's a problem with your upstream process. You'd better fix it.
Comment: Error in openning FastQC
by
friszd
• 0
after running fastqc -o output.html input.fastq, terminal returns: " Specified output directory 'output.html' does not exist " , when i run…
Comment: Is it reasonable to discard reads that show variation of quality across its leng
by
GenoMax
140k
The question you should be looking into is why the Q scores are dropping there? Are there `N` calls indicating some issue with that cycle?
Comment: Error in openning FastQC
by
GenoMax
140k
What is `ctrl+c ctrl+v`? You may actually be killing the running fastqc process. If you run `fastqc` interactively (like what was shown ab…
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Dave Carlson
★ 1.7k
Ugh. I've definitely done this one before as well. And was none too happy about it.
Comment: Error in openning FastQC
by
friszd
• 0
fastqc -o output.html input.fastq ctrl+c ctrl+v
Comment: Error running local blastn in R using system2
by
GenoMax
140k
What do you see if you `cat nt_euk.nal`? Do the number of pieces mentioned in that file match with what you locally have?
Comment: Low number of both surviving reads after trimming
by
GenoMax
140k
It is your data and if you want to do that it is up to you. You may be throwing away essentially good data (you should remove the nextera s…
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Istvan Albert
100k
Some things never change ... the most common mistake is still the one on top there
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