How to generate .sra files from Fastq
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10.4 years ago
ktsuttlemyre ▴ 40

I have been downloading a lot of NCBI short read archive files (.sra files) and I know how to convert them to bam, sam, fastq, etc but is there a way to generate my own .sra files? The tools in the SRA toolkit do not seem to convert to .sra

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fastq ncbi sequence sra • 6.6k views
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Out of curiosity: what would you like to do with *.sra files when you already have the *.bam/*.sam/*.fastq files?

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10.4 years ago

I have never used it but Mothur has a command "make.sra".

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10.4 years ago

You would use one of the X-load tools from the SRA toolkit, where X is bam, fastq, etc. Now why you would actually want to do this is beyond me. You're better off sticking to BAM and fastq, which are actually normal formats used by tools.

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