Hi, I am running a de novo assembly of a plant ~500Mb genome from PacBio reads of 120X coverage with Falcon. It runs for about 5 days now, done ~20% of the 160000 jobs that it has, and the disc space it takes is 2.5T currently. My free disc is up to 6T, meaning that I have 3.5T free. I am trying to estimate the size that I will need. What is the behaviour of Falcon regarding the temp files that it creates and how much free space on a disc it will need...? Is it something that can be calculated?
Oof, 34h to finish a run compared to 5days to 20% of the run, that's some nice improvements :) Glad you found a solution !
Also, just in case someone needs - here are most of the answers to my questions :
https://github.com/PacificBiosciences/FALCON/wiki/Somethings-to-think-about-for-tuning-assembly-parameters
Important text for newbies like me assembling de novo for the first time.