Hi all,
Is it possible to recombine several old scRNA seq studies and answer a biological question i.e. build up a new analysis on the basis of combine analysis of old.
EXAMPLE -I found many single cell RNA seq studies which focus on different tissues.
Can I combine them all to check gene expression of geneX in the different tissues showing relative expression in them.Is it a possible insilico study?
Will there not be a lot of batch effect?If so how can it be avoided?
Will I have to start from gene count or download SRR so that all different scrnaseq studies are quality controlled in the same manner?
Kindly guide.
Thank you so much for the detailed reply and great suggestions.Very helpful !