Entering edit mode
10 months ago
Carolina
•
0
We conducted total mRNA RNA-seq using Illumina technology and encountered significant disparity in the mapping between R2 and R1. We are considering potential issues with trimming; we utilized FastQC and Fastp. We are exploring other possibilities
You should provide the code you ran otherwise it will be difficult to help.
Do not align paired-end data independently, if that is what you doing.