Why does the Pair-End RNA library exhibit a significant disparity between the mapping results of R2 compared to R1?
0
0
Entering edit mode
10 months ago
Carolina • 0

We conducted total mRNA RNA-seq using Illumina technology and encountered significant disparity in the mapping between R2 and R1. We are considering potential issues with trimming; we utilized FastQC and Fastp. We are exploring other possibilities

illumina pair-end r2 rna • 565 views
ADD COMMENT
0
Entering edit mode

You should provide the code you ran otherwise it will be difficult to help.

ADD REPLY
0
Entering edit mode

Do not align paired-end data independently, if that is what you doing.

ADD REPLY

Login before adding your answer.

Traffic: 1345 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6