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116,992 results • Page
1 of 2340
Sort: Rank
Rank
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Votes
Replies
0
votes
0
replies
14
views
constructing pangenome through psvcp
psvcp
pangenome
52 minutes ago by
analyst
▴ 50
0
votes
2
replies
25
views
zero TPM for many gene including housekeeping when using kallisto
RNAseq
updated 10 minutes ago by
ATpoint
82k • written 1 hour ago by
ashkan
▴ 160
0
votes
1
reply
19
views
What marks a De-Novo Genome assembly as FAILED?
nanopore
denovo
illumina
assembly
genome
updated just now by
dthorbur
★ 2.0k • written 1 hour ago by
Umer
▴ 50
0
votes
1
reply
85
views
How to handle duplicated genes in TCGA data?
TCGA
GDC
mRNA
updated 2 hours ago by
txema.heredia
▴ 130 • written 10 hours ago by
Ngrin
• 0
3
votes
7
replies
300
views
Faster Needleman-Wunsch rapid global alignment of two sequences?
Needleman-Wunsch
alignment
updated 3 hours ago by
DGTool
▴ 20 • written 21 hours ago by
Gabriel R.
★ 2.9k
0
votes
2
replies
111
views
How to generate table_annovar from VCF input?
vcf
annovar
updated 2 hours ago by
Pierre Lindenbaum
161k • written 9 hours ago by
melissachua90
▴ 70
0
votes
0
replies
40
views
How do I quantify the non-coding transcripts that I have generated after using CPAT,PLEK, and BLAST altogether?
transcript
Quantify
non-coding
4 hours ago by
Varsha
• 0
797
votes
167
replies
143k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.8k • written 7.4 years ago by
Istvan Albert
100k
0
votes
1
reply
100
views
solution-Transcriptome.cpp:18:Transcriptome: exiting because of *INPUT FILE* error: could not open input file /geneInfo.tab #2142
STAR
updated 21 minutes ago by
GenoMax
142k • written 8 hours ago by
huxiangyulove
• 0
0
votes
0
replies
61
views
Help interpreting KEGG module definitions for converting to NetworkX graph
definition
kegg
module
database
genomics
11 hours ago by
O.rka
▴ 720
4
votes
5
replies
4.3k
views
Tool:
GRIDSS: the Genomic Rearrangement IDentification Software Suite
Assembly
Structural-Variation
Variant-Calling
updated 11 hours ago by
dario.garvan
▴ 520 • written 7.2 years ago by
d-cameron
★ 2.9k
0
votes
0
replies
63
views
Help! Reconstruction of locus, order contigs
contigs
cluster
alignment
locus
11 hours ago by
BATMAN
• 0
0
votes
4
replies
210
views
Merge clusters in Seurat UMAP
seurat
umap
2 hours ago by
kilcdincer
▴ 10
0
votes
0
replies
65
views
Use of ichor CNA
Dog
CNA
genome
16 hours ago by
sainavyav22
• 0
2
votes
2
replies
171
views
Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
single-cell
ambient-RNA
updated 14 hours ago by
Ram
43k • written 17 hours ago by
txema.heredia
▴ 130
0
votes
0
replies
79
views
News:
Course on Manual Genome Curation
Genome-Assembly
Pretext-View
Manual-Genome-Curation
updated 11 hours ago by
Ram
43k • written 18 hours ago by
carlopecoraro2
★ 2.5k
3
votes
2
replies
178
views
Details on salmon index
Salmon
updated 18 hours ago by
Rob
6.6k • written 1 day ago by
Lorenzo
• 0
0
votes
0
replies
72
views
Error when running create-maf-vcf at convertGVCFToHVCFForChrom step - PHGv2
PHG
pangenome
PHG_v2
19 hours ago by
Jsarria.EEAD
• 0
0
votes
1
reply
120
views
How can I calculate the OS of each patient?
overall-survival
updated 19 hours ago by
ATpoint
82k • written 19 hours ago by
Pedro
• 0
0
votes
1
reply
123
views
mitochondrial genome assembly pipeline
mammals
mitochondrial-genome
updated 19 hours ago by
Ram
43k • written 22 hours ago by
m90
▴ 30
0
votes
0
replies
72
views
cat-bgen fail
bgenix
updated 19 hours ago by
Ram
43k • written 20 hours ago by
lambard
• 0
3
votes
4
replies
248
views
Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer version?
RNA-seq
genome
updated 19 hours ago by
Ram
43k • written 1 day ago by
Ali
• 0
3
votes
3
replies
166
views
getting exon coordinates
genomics
updated 20 hours ago by
Ram
43k • written 21 hours ago by
Xbox_27
• 0
0
votes
4
replies
240
views
How to extract cells of different species after mapping with combined genome?
snRNA-seq
scRNA-seq
updated 5 hours ago by
Tony
• 0 • written 1 day ago by
vk
▴ 10
1
vote
5
replies
224
views
Can I perform a correlation test with 3 biological replicates per condition?
RNA-Seq
deseq2
updated 11 hours ago by
Ram
43k • written 1 day ago by
manuelmourato25
• 0
3
votes
4
replies
333
views
finding evidence(s) of a peptide translated from an "Upstream Open Reading Frame (uORF)"
UTR
masspec
uORF
peptide
updated 21 hours ago by
GenoMax
142k • written 1 day ago by
Pierre Lindenbaum
161k
2
votes
11
replies
603
views
DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts in whole blood: is it normal to always get NS?
rstudio
DEG
limma
3 hours ago by
egascon
• 0
1
vote
1
reply
126
views
API kegg - IndexError: list index out of range
kegg
API
updated 7 hours ago by
Nyksubuz
▴ 20 • written 1 day ago by
mirwa.zidi93
• 0
0
votes
0
replies
78
views
Blog:
The Current State and Future Prospects of Long-Read Sequencing
LRS
1 day ago by
Novogene
▴ 420
0
votes
0
replies
82
views
qPCR gene expression data analysis
qPCR
geneexpression
anova
1 day ago by
ebertomeup
• 0
0
votes
3
replies
198
views
Sam file Header problem
Sam
Header
problem
file
updated 3 hours ago by
Pierre Lindenbaum
161k • written 1 day ago by
saifulislam99121
• 0
1
vote
4
replies
212
views
Tool:
Converting Degree Minutes (DM) to Decimal Degrees (DD) using python script
Minutes
Decimal
offtopic
Degrees
python
updated 11 hours ago by
Ram
43k • written 1 day ago by
kuttibiotech2009
▴ 30
0
votes
1
reply
542
views
scRNAseq quality control weird double curve in nFeature vs nCount plot
scRNA-seq
QC
single
cell
BDRhapsody
analysis
updated 1 day ago by
valdirbarth
▴ 20 • written 12 months ago by
Salomé
• 0
0
votes
0
replies
93
views
select set of intervals that cover a genomic region
GRanges
bed
1 day ago by
ntsopoul
▴ 60
1
vote
1
reply
136
views
Tool:
Collect dispersed executable files and run them by using bingo.
bingo
updated 11 hours ago by
Ram
43k • written 1 day ago by
dwpeng
▴ 10
0
votes
1
reply
118
views
BiomartException: Query ERROR for existing dataset in BioMart
biomart
ensembl
wheat
python
pybiomart
updated 5 hours ago by
Nyksubuz
▴ 20 • written 1 day ago by
Luqman
• 0
2
votes
3
replies
198
views
ggrepel function: geom_text_repel()
ggplot2
ggrepel
R
updated 11 hours ago by
Ram
43k • written 1 day ago by
sooni
▴ 20
0
votes
2
replies
154
views
how to treat the replicates while performing WGCNA
clustering
WGCNA
replicates
1 day ago by
mavy
▴ 10
1
vote
1
reply
212
views
Calculate allelic frequency from VEP output vcf file
VEP
variant
1 day ago by
ramiro.barrantes
• 0
1
vote
4
replies
297
views
In one PCA plot, can I calculate the percentage of different factors that contribute to the PCA?
PCA
R
VARIANCE
18 hours ago by
diqixiaoyaoer
▴ 10
2
votes
6
replies
1.9k
views
How can you *consistently* download BioProject IDs from NCBI's BioSample database using Entrez Direct?
XML
ncbi
entrez-direct
updated 1 day ago by
Ram
43k • written 3.9 years ago by
millere
• 0
0
votes
1
reply
148
views
What's next after GATK variant calling pipeline?
GATK
pipeline
figures
DNA-seq
updated 1 day ago by
Michael
54k • written 1 day ago by
mgranada3
▴ 30
0
votes
1
reply
134
views
Add line under stat test
stats
R
updated 7 hours ago by
Nyksubuz
▴ 20 • written 1 day ago by
Ghada
• 0
1
vote
5
replies
784
views
Converting IGV to Figure using Illustrator (or other tools)
Illustrator
IGV
written 6 months ago by
Daniel
▴ 30
0
votes
0
replies
99
views
DOCK6 installation on MacOS M1
installation
troubleshooting
DOCK6
updated 1 day ago by
GenoMax
142k • written 1 day ago by
Rodolfo Adrián
• 0
1
vote
3
replies
189
views
Genotyping sites with N in reference genome
GATK
UnifiedGenotyper
updated 1 day ago by
Zhenyu Zhang
★ 1.2k • written 1 day ago by
shpak.max
▴ 50
2
votes
4
replies
256
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 1 day ago by
yura.grabovska
▴ 90 • written 1 day ago by
ev97
▴ 20
1
vote
14
replies
596
views
Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL > pointer.
blast
ncbi
4 hours ago by
hophuquy0944
• 0
0
votes
4
replies
231
views
Sequencing Depth (Read Depth) Calculations
depth
1 day ago by
LucisTheFather
• 0
0
votes
1
reply
136
views
Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
annotation
vcf
vep
zygosity
deepvariant
1 day ago by
asalimih
▴ 60
116,992 results • Page
1 of 2340
Recent Votes
Answer: Generating consensus sequence from bam file
Answer: Short Read Data Genome Assembly
Comment: API kegg - IndexError: list index out of range
Comment: How does FindNeighbors() and FindClusters() related and work?
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
lncRNA and ncPEPs identification
Answer: Identifying common DEGs among multiple datasets
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Recent Replies
Comment: What marks a De-Novo Genome assembly as FAILED?
by
dthorbur
★ 2.0k
I'm a little confused about your experimental design. Are you making 45 different assemblies? Or are all the samples from the same individu…
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ATpoint
82k
You should be stating how you constructed the index, and include the actual lines for the problematic genes from the abundance files. Note …
Comment: zero TPM for many gene including housekeeping when using kallisto
by
dariober
14k
You could try [salmon](https://combine-lab.github.io/salmon/getting_started/) to see if the problem is with kallisto or with the pseudo-ali…
Comment: solution-Transcriptome.cpp:18:Transcriptome: exiting because of *INPUT FILE* err
by
GenoMax
142k
> INPUT FILE error: could not open input file /geneInfo.tab Based on the error it appears that the input file you are providing could not …
Answer: How to handle duplicated genes in TCGA data?
by
txema.heredia
▴ 130
Having duplicate gene symbols is pretty common. Why do you need to work with gene symbols? To compare them to external datasets? Or just fo…
Comment: Merge clusters in Seurat UMAP
by
kilcdincer
▴ 10
It did not help either. But I got the answer [here][1] Thank you! [1]: https://github.com/satijalab/seurat/issues/8902
Comment: How to generate table_annovar from VCF input?
by
Pierre Lindenbaum
161k
there is no `--allsample` option in the "usage" screen you're showing. I suspect you're using an old version ?
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
by
egascon
• 0
Thank you very much again. I have learned a lot of things.
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
by
egascon
• 0
It's a microarryay experiment, not RNASeq
Comment: Sam file Header problem
by
Pierre Lindenbaum
161k
https://gatk.broadinstitute.org/hc/en-us/articles/360035890671-Read-groups
Answer: Faster Needleman-Wunsch rapid global alignment of two sequences?
by
DGTool
▴ 20
I have also recently come across another global alignment algorithm that was recently released which could seem pretty interesting to test …
Comment: Faster Needleman-Wunsch rapid global alignment of two sequences?
by
b.contreras.moreira
▴ 190
Not sure, you might ask in the repo, please share here the response if you find it
Comment: How to generate table_annovar from VCF input?
by
LucisTheFather
• 0
Would you be more specific to your question? Do you mean how to debug this syntax error?
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
hophuquy0944
• 0
thank you
Comment: How to extract cells of different species after mapping with combined genome?
by
Tony
• 0
I think this method should be applicable to all high-throughput single-cell RNA-seq platforms. Although the method of determining cell spec…
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