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1,000 results • Page 1 of 20
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5
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4
replies
314
views
Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
1 hour ago by Estevão • 0
0
votes
0
replies
171
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
9 days ago by yxwucq • 0
4
votes
2
replies
312
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 16 days ago by 43k • written 16 days ago by ▴ 10
7
votes
7
replies
1.5k
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 16 days ago by ★ 2.5k • written 17 days ago by • 0
1
vote
2
replies
354
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
15 days ago by Javier • 0
16
votes
10
replies
1.1k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 4 weeks ago by 5.3k • written 4 weeks ago by ★ 2.4k
1
vote
1
reply
224
views
Forum: Can I use this community for software development?
software-development
updated 5 weeks ago by 43k • written 5 weeks ago by • 0
5
votes
2
replies
391
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 5 weeks ago by 54k • written 5 weeks ago by • 0
0
votes
0
replies
177
views
Forum: Field of Study in Computational Biology
Genomics Computational-Biology PhD
updated 6 weeks ago by 43k • written 6 weeks ago by • 0
1
vote
3
replies
484
views
Forum: Development plan for a bioinformatics database developer/coder
developer
6 weeks ago by pixie@bioinfo ★ 1.5k
7
votes
8
replies
705
views
Forum: Bioinformatics career transition
career forum
updated 6 weeks ago by ★ 16k • written 6 weeks ago by ▴ 20
4
votes
2
replies
487
views
Forum: Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein CAFA5
7 weeks ago by Anatoly A. ▴ 10
6
votes
6
replies
779
views
Forum: You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical thought-experiment
8 weeks ago by jared.andrews07 ★ 16k
0
votes
0
replies
165
views
Forum: What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc tcga genomics-data-commons
updated 8 weeks ago by 43k • written 8 weeks ago by ★ 1.2k
0
votes
2
replies
387
views
Forum: What's the state of the art in bacterial whole genome alignment?
whole genome alignment mauve
8 weeks ago by Dave Carlson ★ 1.7k
31
votes
12
replies
1.0k
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
9 weeks ago by karlaarz ▴ 90
5
votes
4
replies
567
views
Forum: Would reaching out to local labs and startups help with my career transition?
career volunteering
updated 9 weeks ago by 142k • written 9 weeks ago by ▴ 10
1
vote
2
replies
374
views
Forum: Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity Career-Gap career-reentry
updated 10 weeks ago by 82k • written 10 weeks ago by ▴ 20
8
votes
7
replies
818
views
6 follow
Forum: Tower Server configuration for NGS data analysis
NGS
updated 10 weeks ago by ▴ 120 • written 3 months ago by • 0
1
vote
0
replies
282
views
Forum: list of bioinformatics tools in cancer genomics
mutations CNA cancer genomics LOH
updated 10 weeks ago by 161k • written 10 weeks ago by ★ 1.4k
4
votes
2
replies
382
views
Forum: bulk seq vs. sc-seq
scATAC-seq ATAC-seq scRNAseq RNAseq
updated 12 weeks ago by ★ 5.9k • written 12 weeks ago by ▴ 10
8
votes
6
replies
2.8k
views
Forum: Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by 10k
0
votes
0
replies
204
views
Forum: tutorial for carrier screening analyses?
carrier-screening
updated 3 months ago by 43k • written 3 months ago by ▴ 20
2
votes
3
replies
437
views
Forum: My Project
Project CLI MD-Simulations Molecular-docking
3 months ago by Ajay A Shettigar • 0
17
votes
11
replies
998
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
10
votes
7
replies
2.2k
views
Forum: How to learn bioinformatics?
learning
updated 3 months ago by ★ 1.3k • written 23 months ago by ▴ 20
26
votes
27
replies
2.1k
views
10 follow
Forum: Determining computer time required
computer hpc time
updated 3 months ago by ★ 5.9k • written 3 months ago by ▴ 40
7
votes
6
replies
740
views
Forum: where to work as a bioinformatician (NGS/IA)
employment omics ia ngs
updated 3 months ago by 43k • written 3 months ago by ▴ 20
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
2
votes
1
reply
436
views
Forum: Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist transcriptomics nanostring xenium visium
3 months ago by LauferVA 4.2k
49
votes
20
replies
2.0k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 3 months ago by ★ 27k
4
votes
3
replies
780
views
Forum: How to get started in bioinformatics
students
updated 3 months ago by 43k • written 4 months ago by ▴ 10
2
votes
2
replies
586
views
Forum: Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat single-cell omics batch rnaseq
updated 4 months ago by 43k • written 4 months ago by ▴ 40
0
votes
2
replies
3.2k
views
Forum: I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by 142k • written 4 months ago by ▴ 10
5
votes
3
replies
614
views
Forum: Binning the rare biosphere
metagenomics soil assembly techniques binning
updated 4 months ago by ★ 27k • written 4 months ago by ▴ 20
6
votes
2
replies
668
views
Forum: how to start Bioinformatics?
basics
updated 4 months ago by ★ 27k • written 4 months ago by • 0
19
votes
5
replies
1.1k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
4 months ago by Raony Guimarães ★ 1.4k
4
votes
6
replies
932
views
6 follow
Forum: Bioinformatics: what do you think of it in the next 20 years?
future
updated 5 months ago by 43k • written 5 months ago by • 0
1
vote
1
reply
492
views
Forum: want to work in open source project/project in bioinformatics
project
updated 4 months ago by 43k • written 5 months ago by • 0
3
votes
5
replies
1.0k
views
Forum: Current Trends in Bioinformatics
structural-bioinformatics
updated 4 months ago by 43k • written 5 months ago by • 0
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 14 months ago by ▴ 150
176
votes
46
replies
19k
views
26 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by Istvan Albert 100k
3
votes
2
replies
626
views
Forum: RNA-Seq data analysis - question
Python RNA-Seq R
updated 5 months ago by ▴ 10 • written 5 months ago by ▴ 40
0
votes
0
replies
371
views
Forum: How to learn bioinformatics online?
self-education education learning
updated 6 months ago by 43k • written 6 months ago by • 0
6
votes
5
replies
2.3k
views
Forum: Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by tshtatland ▴ 190
7
votes
9
replies
1.4k
views
6 follow
Forum: Mentor for a beginner bioinformatics student
project mentor
6 months ago by UT_FAN ▴ 40
0
votes
0
replies
460
views
Forum: Steps after doublet detection in single cell data
scRNA doublet
6 months ago by ishwarvh • 0
0
votes
0
replies
357
views
Forum: Automated Checks And QA
retrospective
6 months ago by Sasha ▴ 840
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 7 months ago by ▴ 10
21
votes
7
replies
11k
views
Forum: Bioinformatics short paper journal resource [software/webapps/database]
journal software webapp
updated 7 months ago by ▴ 100 • written 8.1 years ago by 11k
1,000 results • Page 1 of 20
Recent Votes
Comment: Bacterial plasmid analysis
Answer: Problem with data downloaded from Short Reads Archive (SRA)
Answer: Super ehancers
Comment: Bacterial plasmid analysis
Answer: Why employ normalization methods, and how can they be utilized in DEG analysis?
Answer: Why employ normalization methods, and how can they be utilized in DEG analysis?
Answer: What analysis suitable to identify similarly expressed genes between two samples
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Recent Replies
Answer: Loss of 'var' using concatenation of AnnData objects by Hugo • 0
Similar question has been asked and answered on [scverse discourse](https://discourse.scverse.org/t/loosing-anndata-var-layer-when-using-sc…
Comment: Bacterial plasmid analysis by Zamin Iqbal ▴ 20
Hi Nicole. 1. The clustering will be quite different between pling and the rep types (because plasmids with one rep type can still be ve…
Comment: Do I need to go back and filter my long-reads? by Ram 43k
Do not delete posts that have received feedback. Engage with the user providing you feedback.
Answer: Download eQTL data of one specific gene for all tissues from GTEx by bk11 ★ 2.4k
Please kindly check your result here. You can click `csv` and download the date in this [link][1]. **OR** If you prefer to use R, there i…
Comment: RNAseq one control two conditions, shared and exclusive genes by matteo.levorato • 0
but why not a single matrix with the 3 different condition and then by lfcShrink function get the DEGs of each condition?
Answer: Super ehancers by jared.andrews07 ★ 16k
Sure, you can run it on them. Stitching tends to happen less frequently since the ATAC peaks are generally less wide than H3K27ac, but it'l…
Answer: What purposes can TPM values be used for? by jared.andrews07 ★ 16k
TPMs cannot be used for intersample comparisons, but they can be used for intrasample comparisons (e.g. comparing relative expression level…
Comment: Bacterial plasmid analysis by nicole.kavanagh • 0
Thank you so much for the recommendation and the very clear explanation , it's much appreciated. I have already made core-gene phylogenies/…
Comment: What purposes can TPM values be used for? by Adam ▴ 30
Have you run FASTQC and checked the mapping efficiency of WT3? > would running DESeq using TPM values help solve my problem? No, DESeq sh…
Answer: How goes gene length effect the number of reads mapped by i.sudbery 19k
See my answer about the what hows and whens of RNA-seq normalisation here: https://www.biostars.org/p/9586553/#9586566
Comment: What purposes can TPM values be used for? by dsull ★ 5.9k
Since you have mutant vs wild-type, using DESeq2 is the right thing to do. Rather than showing a small table of numbers, you should make a …
Answer: What purposes can TPM values be used for? by i.sudbery 19k
See my post here for more details on RNA-seq normalisation: https://www.biostars.org/p/9586553/#9586566 In short, TPM normalises for ge…
Comment: Bacterial plasmid analysis by Zamin Iqbal ▴ 20
I vote for this, it's the best! (but also, I am Daria's PhD supervisor so I am biased). Preprint out soon(ish) [no pressure Daria!]
Comment: Downsampling long-read BAM files by GenoMax 142k
Is this what you used ultimately? Did this tool satisfy the "specific average depth" requirement? You could accept (green check mark) this…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory by Estevão • 0
Thank you. My budget is around 20 thousand dollars.
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