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385 results • Page 1 of 8
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6
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4
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438
views
Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
14 days ago by Estevão ▴ 10
0
votes
0
replies
193
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
24 days ago by yxwucq • 0
5
votes
2
replies
365
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 4 weeks ago by 43k • written 4 weeks ago by ▴ 20
7
votes
7
replies
1.9k
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 4 weeks ago by ★ 2.5k • written 4 weeks ago by • 0
1
vote
2
replies
404
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
29 days ago by Javier • 0
16
votes
10
replies
1.2k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 6 weeks ago by 5.3k • written 7 weeks ago by ★ 2.4k
1
vote
1
reply
244
views
Forum: Can I use this community for software development?
software-development
updated 7 weeks ago by 43k • written 7 weeks ago by • 0
5
votes
2
replies
407
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 7 weeks ago by 54k • written 7 weeks ago by • 0
0
votes
0
replies
193
views
Forum: Field of Study in Computational Biology
Genomics Computational-Biology PhD
updated 8 weeks ago by 43k • written 8 weeks ago by • 0
1
vote
3
replies
510
views
Forum: Development plan for a bioinformatics database developer/coder
developer
9 weeks ago by pixie@bioinfo ★ 1.5k
7
votes
8
replies
773
views
Forum: Bioinformatics career transition
career forum
updated 9 weeks ago by ★ 16k • written 9 weeks ago by ▴ 20
4
votes
2
replies
530
views
Forum: Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein CAFA5
9 weeks ago by Anatoly A. ▴ 10
6
votes
6
replies
809
views
Forum: You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical thought-experiment
10 weeks ago by jared.andrews07 ★ 16k
0
votes
0
replies
194
views
Forum: What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc tcga genomics-data-commons
updated 10 weeks ago by 43k • written 10 weeks ago by ★ 1.2k
0
votes
2
replies
430
views
Forum: What's the state of the art in bacterial whole genome alignment?
whole genome alignment mauve
10 weeks ago by Dave Carlson ★ 1.7k
33
votes
12
replies
1.1k
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
11 weeks ago by karlaarz ▴ 110
5
votes
4
replies
605
views
Forum: Would reaching out to local labs and startups help with my career transition?
career volunteering
updated 12 weeks ago by 142k • written 12 weeks ago by ▴ 10
1
vote
2
replies
404
views
Forum: Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity Career-Gap career-reentry
updated 12 weeks ago by 82k • written 12 weeks ago by ▴ 20
8
votes
7
replies
866
views
6 follow
Forum: Tower Server configuration for NGS data analysis
NGS
updated 12 weeks ago by ▴ 120 • written 3 months ago by • 0
1
vote
0
replies
306
views
Forum: list of bioinformatics tools in cancer genomics
mutations CNA cancer genomics LOH
updated 12 weeks ago by 161k • written 12 weeks ago by ★ 1.4k
4
votes
2
replies
405
views
Forum: bulk seq vs. sc-seq
scATAC-seq ATAC-seq scRNAseq RNAseq
updated 3 months ago by ★ 6.0k • written 3 months ago by ▴ 10
8
votes
6
replies
2.9k
views
Forum: Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by 10k
0
votes
0
replies
217
views
Forum: tutorial for carrier screening analyses?
carrier-screening
updated 3 months ago by 43k • written 3 months ago by ▴ 20
2
votes
3
replies
456
views
Forum: My Project
Project CLI MD-Simulations Molecular-docking
3 months ago by Ajay A Shettigar • 0
17
votes
11
replies
1.0k
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
10
votes
7
replies
2.3k
views
Forum: How to learn bioinformatics?
learning
updated 3 months ago by ★ 1.3k • written 24 months ago by ▴ 20
26
votes
27
replies
2.2k
views
10 follow
Forum: Determining computer time required
computer hpc time
updated 4 months ago by ★ 6.0k • written 4 months ago by ▴ 40
7
votes
6
replies
772
views
Forum: where to work as a bioinformatician (NGS/IA)
employment omics ia ngs
updated 4 months ago by 43k • written 4 months ago by ▴ 20
21
votes
12
replies
3.6k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
4 months ago by Ram 43k
2
votes
1
reply
462
views
Forum: Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist transcriptomics nanostring xenium visium
4 months ago by LauferVA 4.2k
49
votes
20
replies
2.1k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 4 months ago by ★ 2.6k • written 4 months ago by ★ 27k
4
votes
3
replies
814
views
Forum: How to get started in bioinformatics
students
updated 4 months ago by 43k • written 4 months ago by ▴ 10
2
votes
2
replies
604
views
Forum: Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat single-cell omics batch rnaseq
updated 4 months ago by 43k • written 4 months ago by ▴ 40
0
votes
2
replies
3.3k
views
Forum: I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by 142k • written 4 months ago by ▴ 10
5
votes
3
replies
645
views
Forum: Binning the rare biosphere
metagenomics soil assembly techniques binning
updated 5 months ago by ★ 27k • written 5 months ago by ▴ 20
6
votes
2
replies
692
views
Forum: how to start Bioinformatics?
basics
updated 5 months ago by ★ 27k • written 5 months ago by • 0
19
votes
5
replies
1.2k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
4 months ago by Raony Guimarães ★ 1.4k
4
votes
6
replies
971
views
6 follow
Forum: Bioinformatics: what do you think of it in the next 20 years?
future
updated 5 months ago by 43k • written 5 months ago by • 0
1
vote
1
reply
510
views
Forum: want to work in open source project/project in bioinformatics
project
updated 5 months ago by 43k • written 5 months ago by • 0
3
votes
5
replies
1.1k
views
Forum: Current Trends in Bioinformatics
structural-bioinformatics
updated 5 months ago by 43k • written 6 months ago by • 0
21
votes
11
replies
2.6k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 14 months ago by ▴ 150
176
votes
46
replies
19k
views
26 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by Istvan Albert 100k
3
votes
2
replies
644
views
Forum: RNA-Seq data analysis - question
Python RNA-Seq R
updated 6 months ago by ▴ 10 • written 6 months ago by ▴ 40
0
votes
0
replies
389
views
Forum: How to learn bioinformatics online?
self-education education learning
updated 6 months ago by 43k • written 6 months ago by • 0
6
votes
5
replies
2.3k
views
Forum: Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by tshtatland ▴ 190
7
votes
9
replies
1.5k
views
6 follow
Forum: Mentor for a beginner bioinformatics student
project mentor
6 months ago by UT_FAN ▴ 40
0
votes
0
replies
479
views
Forum: Steps after doublet detection in single cell data
scRNA doublet
7 months ago by ishwarvh • 0
0
votes
0
replies
370
views
Forum: Automated Checks And QA
retrospective
7 months ago by Sasha ▴ 840
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 7 months ago by ▴ 10
21
votes
7
replies
12k
views
Forum: Bioinformatics short paper journal resource [software/webapps/database]
journal software webapp
updated 7 months ago by ▴ 100 • written 8.1 years ago by 11k
385 results • Page 1 of 8
Recent Votes
Answer: In one PCA plot, can I calculate the percentage of different factors that contri
Answer: Details on salmon index
Answer: Generating consensus sequence from bam file
Answer: Short Read Data Genome Assembly
Comment: API kegg - IndexError: list index out of range
Comment: How does FindNeighbors() and FindClusters() related and work?
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
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Recent Replies
Comment: In one PCA plot, can I calculate the percentage of different factors that contri by diqixiaoyaoer ▴ 10
You can have a look on my answer. I have post it below.
Answer: In one PCA plot, can I calculate the percentage of different factors that contri by diqixiaoyaoer ▴ 10
Here is my script that can be used to calculate how much degree that different factors contribute to PCA variance (PC1 and PC2). # …
Comment: Details on salmon index by Lorenzo • 0
Thank you so much for the beautiful explanation. Just one more curiosity. So in the first versions Salmon utilized RapMap to index and then…
Comment: What marks a De-Novo Genome assembly as FAILED? by dthorbur ★ 2.0k
I'm a little confused about your experimental design. Are you making 45 different assemblies? Or are all the samples from the same individu…
Comment: zero TPM for many gene including housekeeping when using kallisto by ATpoint 82k
You should be stating how you constructed the index, and include the actual lines for the problematic genes from the abundance files. Note …
Comment: zero TPM for many gene including housekeeping when using kallisto by dariober 14k
You could try [salmon](https://combine-lab.github.io/salmon/getting_started/) to see if the problem is with kallisto or with the pseudo-ali…
Comment: solution-Transcriptome.cpp:18:Transcriptome: exiting because of *INPUT FILE* err by GenoMax 142k
> INPUT FILE error: could not open input file /geneInfo.tab Based on the error it appears that the input file you are providing could not …
Answer: How to handle duplicated genes in TCGA data? by txema.heredia ▴ 130
Having duplicate gene symbols is pretty common. Why do you need to work with gene symbols? To compare them to external datasets? Or just fo…
Comment: Merge clusters in Seurat UMAP by kilcdincer ▴ 10
It did not help either. But I got the answer [here][1] Thank you! [1]: https://github.com/satijalab/seurat/issues/8902
Comment: How to generate table_annovar from VCF input? by Pierre Lindenbaum 161k
there is no `--allsample` option in the "usage" screen you're showing. I suspect you're using an old version ?
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i by egascon • 0
Thank you very much again. I have learned a lot of things.
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i by egascon • 0
It's a microarryay experiment, not RNASeq
Comment: Sam file Header problem by Pierre Lindenbaum 161k
https://gatk.broadinstitute.org/hc/en-us/articles/360035890671-Read-groups
Answer: Faster Needleman-Wunsch rapid global alignment of two sequences? by DGTool ▴ 20
I have also recently come across another global alignment algorithm that was recently released which could seem pretty interesting to test …
Comment: Faster Needleman-Wunsch rapid global alignment of two sequences? by b.contreras.moreira ▴ 190
Not sure, you might ask in the repo, please share here the response if you find it
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