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5.8 years ago
rob.costa1234
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310
I have an RNAseq data of three time points. Day 1 , 3 and 6. Day 1 cells has a specific marker (MYC) and are enriched in specific pathway. The marker (MYC) and pathways got altered when cell reach Day 6 and accordingly change the cell state. My question is if I have to force / maintain these cells in day 1 what pathways regulate such mechanism that cell remain in day1 state Day 6 state. I have an un-induced control a each time point to calculate DE
Which DE I should be looking at?
Trust me it is not an assignment but a question answer to which I cannot find after reading number of publications.
Even if it was somehow possible by manipulating one or more proteins/pathways, how would you deal with countless other things that are simultaneously changing (some in response to the manipulations you tried). It would be like trying to plug holes in a vessel with just 10 fingers when new ones keep appearing.
Can you explain why the experiment was designed this way and what was the main aim?