Entering edit mode
2.8 years ago
Ric
▴
440
Hello, We have 2 Iso-seq libaryries:
> ls -hlal 1_A01/
total 594K
drwxrws--- 2 330616 _Team 4.0K Jan 10 2018 .
drwxrws--- 4 330616 _Team 4.0K May 24 2018 ..
-rwxrwx--- 1 330616 _Team 60 Dec 4 2017 m54105_171201_020331.adapters.fasta
-rwxrwx--- 1 330616 _Team 259K Dec 4 2017 m54105_171201_020331.baz2bam_1.log
-rwxrwx--- 1 330616 _Team 14K Dec 4 2017 .m54105_171201_020331.metadata.xml
-rwxrwx--- 1 330616 _Team 51K Dec 4 2017 .m54105_171201_020331.run.metadata.xml
-rwxrwx--- 1 330616 _Team 23G Dec 4 2017 m54105_171201_020331.scraps.bam
-rwxrwx--- 1 330616 _Team 30M Dec 4 2017 m54105_171201_020331.scraps.bam.pbi
-rwxrwx--- 1 330616 _Team 105K Dec 4 2017 m54105_171201_020331.sts.xml
-rwxrwx--- 1 330616 _Team 7.9G Dec 4 2017 m54105_171201_020331.subreads.bam
-rwxrwx--- 1 330616 _Team 16 Jan 10 2018 m54105_171201_020331.subreads.bam.bai
-rwxrwx--- 1 330616 _Team 21M Dec 4 2017 m54105_171201_020331.subreads.bam.pbi
-rwxrwx--- 1 330616 _Team 16K Dec 4 2017 m54105_171201_020331.subreadset.xml
-rwxrwx--- 1 330616 _Team 535 Dec 4 2017 m54105_171201_020331.transferdone
-rwxrwx--- 1 330616 _Team 117K Dec 4 2017 tmp-file-7db72207-6fb3-415d-bfc1-34023b7b6837.txt
> ls -hlal 2_B01/
total 598K
drwxrws--- 2 330616 _Team 4.0K Dec 4 2017 .
drwxrws--- 4 330616 _Team 4.0K May 24 2018 ..
-rwxrwx--- 1 330616 _Team 60 Dec 4 2017 m54105_171201_121745.adapters.fasta
-rwxrwx--- 1 330616 _Team 268K Dec 4 2017 m54105_171201_121745.baz2bam_1.log
-rwxrwx--- 1 330616 _Team 14K Dec 4 2017 .m54105_171201_121745.metadata.xml
-rwxrwx--- 1 330616 _Team 51K Dec 4 2017 .m54105_171201_121745.run.metadata.xml
-rwxrwx--- 1 330616 _Team 23G Dec 4 2017 m54105_171201_121745.scraps.bam
-rwxrwx--- 1 330616 _Team 37M Dec 4 2017 m54105_171201_121745.scraps.bam.pbi
-rwxrwx--- 1 330616 _Team 105K Dec 4 2017 m54105_171201_121745.sts.xml
-rwxrwx--- 1 330616 _Team 11G Dec 4 2017 m54105_171201_121745.subreads.bam
-rwxrwx--- 1 330616 _Team 28M Dec 4 2017 m54105_171201_121745.subreads.bam.pbi
-rwxrwx--- 1 330616 _Team 16K Dec 4 2017 m54105_171201_121745.subreadset.xml
-rwxrwx--- 1 330616 _Team 535 Dec 4 2017 m54105_171201_121745.transferdone
-rwxrwx--- 1 330616 _Team 117K Dec 4 2017 tmp-file-990e0835-a96d-4bf3-a641-0cf498e7f112.txt
- How can I map the Iso-seq data against our genome?
- How can use the iso-seq data for gene prediction?
Thank you in advance,
Have you tried googling this before asking? If so, what are the options you are considering?