Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
1,000 results • Page
1 of 20
Sort: replies
Rank
Views
Votes
Replies
41
votes
55
replies
12k
views
6 follow
hash function for DNA sequences
hash
hashing
DNA
8.1 years ago by
midox
▴ 290
4
votes
45
replies
7.9k
views
SAM file wrong? help with validatesamfile
EXOME
updated 13 months ago by
Ram
43k • written 7.9 years ago by
cristina_sabiers
▴ 110
19
votes
43
replies
5.6k
views
Annotation of huge number of CNV files
CNV annotation TCGA
5.7 years ago by
nazaninhoseinkhan
▴ 520
16
votes
38
replies
17k
views
Differential gene Analysis by Limma
Limma
Microarray
R
updated 2.1 years ago by
Ram
43k • written 9.2 years ago by
Mo
▴ 920
17
votes
37
replies
4.8k
views
No alias or index file found for protein database
next-gen
software error
sequence
updated 5.0 years ago by
Biostar
20 • written 5.1 years ago by
williamsbrian5064
▴ 520
5
votes
35
replies
3.8k
views
Reduce set of chromosomes in Pangenome graph
pangenome
vg
updated 10 months ago by
Jordan M Eizenga
▴ 460 • written 11 months ago by
anivlete
• 0
3
votes
32
replies
3.5k
views
fastqc report for degradome reads
fastqc
adaptor
6.1 years ago by
Sam
▴ 150
8
votes
30
replies
5.3k
views
Per Base Sequence Content
sequencing
fastqc
genome
sequence
updated 4.2 years ago by
wm
▴ 560 • written 4.2 years ago by
Researcher
▴ 20
4
votes
30
replies
2.4k
views
Script makes different file then the manual command, but the command is the same
pipeline
linux
awk
3.9 years ago by
stan.aanhane
▴ 30
14
votes
30
replies
3.4k
views
Download GTF/GFF annotation data for NT database, not by organism (for STAR alignment)
RNA-Seq
rna-seq
alignment
next-gen
5.0 years ago by
Malka
▴ 80
4
votes
30
replies
2.5k
views
SNPs and DEL/MNP in the same position. (DEL or SNP ??)
snp
indels
vcf
calls
mpileup
updated 3.8 years ago by
Ram
43k • written 3.8 years ago by
sami
▴ 40
0
votes
29
replies
3.0k
views
concordance rate between SNP observed and SNP not observed
SNP
updated 5.9 years ago by
Biostar
20 • written 6.1 years ago by
nour.hadjz
▴ 20
11
votes
29
replies
9.9k
views
SummarizedExperiment nrow differs from ncol
DESeq2
SummarizedExperiment
updated 2.6 years ago by
Ram
43k • written 9.9 years ago by
Parham
★ 1.6k
7
votes
29
replies
2.5k
views
What's the powerful biological methods for significant genes selection?
gene selection
significant
biological method
R
5.5 years ago by
Chaimaa
▴ 260
12
votes
28
replies
2.8k
views
Calculating the expression level of genes
Gene expression
RNA-Seq
R
5.9 years ago by
Za
▴ 140
2
votes
28
replies
7.7k
views
Memory use in indexing
Assembly
software error
updated 5.8 years ago by
Ram
43k • written 6.0 years ago by
marongiu.luigi
▴ 710
9
votes
27
replies
7.7k
views
How do I use Glimmer 3.02?
sequencing
updated 5.3 years ago by
ojelizodun
• 0 • written 6.2 years ago by
nattzy94
▴ 50
3
votes
27
replies
5.6k
views
7 follow
Did anyone manage to successfully run HLAScan and get results?
HLA-Typing
NGS
updated 5.8 years ago by
Shiqi Li
• 0 • written 5.9 years ago by
zeynep
▴ 10
4
votes
27
replies
4.4k
views
MISO test run fails error of bam file was not found
RNA-Seq
Assembly
software error
next-gen
genome
updated 3.4 years ago by
Biostar
20 • written 7.7 years ago by
JoeDoasi
▴ 10
3
votes
26
replies
2.0k
views
6 follow
STAR Genome index Error
STAR
updated 7 months ago by
Ram
43k • written 7 months ago by
Prasanna
• 0
1
vote
26
replies
6.3k
views
plink --assoc command gives "NA" for all the analysis (F_A,F_U,A2,CHISQ,P,OR values)
SNP
plink
association
analysis
5.3 years ago by
kushagraprasad24
• 0
10
votes
25
replies
3.6k
views
The interpretation of PCA
DESeq2
R
PCA
updated 5.8 years ago by
Biostar
20 • written 5.9 years ago by
Za
▴ 140
24
votes
25
replies
8.1k
views
How to generate Beta diversity boxplot from phyloseq object?
phyloseq
beta diversity
metagenomics
3.8 years ago by
dpc
▴ 240
8
votes
25
replies
3.1k
views
construction of a database
sql
noSQL
neo4j
database
updated 14 months ago by
Ram
43k • written 3.0 years ago by
Debut
▴ 20
11
votes
24
replies
5.5k
views
BWA mem skip orientation
assembly
genome
alignment
next-gen
5.0 years ago by
williamsbrian5064
▴ 520
0
votes
24
replies
3.5k
views
converting spaces to tabs in gtf files
NCBI
3.0 years ago by
storm1907
▴ 30
13
votes
24
replies
4.7k
views
Error of bam-to-sam conversion using samtools
samtools
TopHat2
RNA-Seq
BAM
SAM
updated 7.2 years ago by
GenoMax
142k • written 7.2 years ago by
Gary
▴ 480
5
votes
24
replies
8.7k
views
Plink1.9 gives error when converting VCF.gz to PED? "Error read failure"
VCF
Plink
5.9 years ago by
DanielC
▴ 170
10
votes
23
replies
2.5k
views
Two simillarly annotated sequence has no alignment similarity. Why?
blast
alignment
gene
updated 6.5 years ago by
GenoMax
142k • written 6.5 years ago by
Farbod
★ 3.4k
1
vote
23
replies
1.8k
views
Parse multifasta files based on a values in two columns in a metadata file
parse
mutlifasta
RefSeq
database
strain
3.7 years ago by
jmwhitha
• 0
1
vote
23
replies
2.1k
views
FASTQ exctract ID's
fastq
4.4 years ago by
User000
▴ 700
6
votes
23
replies
3.0k
views
Could you help me set up a VCF filter?
genome
software error
VCF filter
5.9 years ago by
Charlie2
▴ 50
11
votes
22
replies
4.3k
views
No significant DEG: A request to double check my commands for limma.
limma
differential-gene-expression
updated 5 weeks ago by
Ram
43k • written 5.0 years ago by
RNAseqer
▴ 270
23
votes
22
replies
50k
views
6 follow
Converting VCF to PLINK .bed binary fileset to check for pedigree errors with KING: How do conversion tools make the PLINK .fam file, without asking …
plink
vcf
fam
gatk
vcftools
updated 17 months ago by
Nicole
• 0 • written 6.0 years ago by
gaelgarcia
▴ 270
1
vote
22
replies
3.6k
views
Genome Index and Alignment- STAR
RNA-Seq
genome
alignment
STAR
updated 5.5 years ago by
Kevin Blighe
88k • written 5.5 years ago by
carolgalah
• 0
3
votes
22
replies
3.2k
views
Making RefSeq in Windows
refseq
7.8 years ago by
Alireza Ebadi Tabrizi
• 0
5
votes
22
replies
3.4k
views
Differential analysis between two cell lines
R
RNA-Seq
edgeR
differential analysis
5.9 years ago by
Biologist
▴ 290
0
votes
22
replies
3.4k
views
Using java Treeview to analyse genes: how to select and compile group of genes to present?
RNA-Seq
6.1 years ago by
Muha0216
• 0
4
votes
22
replies
5.7k
views
samtools coverage usage
NGS
updated 2.9 years ago by
Lila M
★ 1.2k • written 2.9 years ago by
smrutimayipanda
▴ 20
5
votes
22
replies
7.5k
views
SVM for classified gene expression data
R
svm
microarray
updated 21 months ago by
Ram
43k • written 8.3 years ago by
Shaurya Jauhari
▴ 50
1
vote
22
replies
6.3k
views
how to use faSplit to split fasta into x files
next-gen
faSplit
sequence
5.5 years ago by
olechnwin
▴ 60
2
votes
22
replies
2.4k
views
6 follow
Find ~1 Mb regions of genome that are shared by two or more WGS samples?
pedigree
linkage
genetics
19 months ago by
Joel Wallenius
▴ 210
13
votes
22
replies
11k
views
SVA : Setting up the data from an ExpressionSet and applying sva function to extimate batches
sva
combat
batch-effect
updated 4 weeks ago by
Ram
43k • written 6.8 years ago by
lessismore
★ 1.3k
2
votes
21
replies
4.2k
views
Compute the correlations between submatrices
correlation
R
matrix
foreach
updated 5.1 years ago by
Ram
43k • written 5.1 years ago by
pablo
▴ 300
16
votes
21
replies
2.3k
views
Why there are different hit for a same gene in different species
gene
blast
ortholog
paralog
7.6 years ago by
Farbod
★ 3.4k
6
votes
21
replies
2.8k
views
How to evaluate the similarity of genes between two sample
RNA-Seq
differential
updated 5.6 years ago by
Biostar
20 • written 5.8 years ago by
afli
▴ 190
1
vote
21
replies
3.0k
views
Center and scale RIN values for DESeq2?
deseq2
rin
3.7 years ago by
randalljellis
▴ 90
3
votes
21
replies
3.8k
views
Normalization agilent microarray data?
Microarray
Normalization
updated 3.0 years ago by
Ram
43k • written 4.7 years ago by
mathavanbioinfo
▴ 90
0
votes
21
replies
2.8k
views
HTSeq-Count: no_feature too high?
htseq-count
18 months ago by
sea.joson
▴ 10
7
votes
21
replies
3.2k
views
Merged.gtf vs. Rnor_6.0.gtf for Raw Counts
RNA-Seq
cuffmerge
raw counts
7.5 years ago by
jmsyl.hong
• 0
1,000 results • Page
1 of 20
Recent Votes
Answer: In one PCA plot, can I calculate the percentage of different factors that contri
Answer: In one PCA plot, can I calculate the percentage of different factors that contri
Answer: Details on salmon index
Answer: Generating consensus sequence from bam file
Answer: Short Read Data Genome Assembly
Comment: API kegg - IndexError: list index out of range
Comment: How does FindNeighbors() and FindClusters() related and work?
Recent Locations •
All
Stockholm, Sweden,
just now
United Kingdom,
just now
Germany,
just now
The Netherlands,
1 minute ago
France,
1 minute ago
Germany,
2 minutes ago
Australia,
4 minutes ago
Recent Awards •
All
Popular Question
to
ashkan
▴ 160
Supporter
to
Umer
▴ 50
Scholar
to
zx8754
11k
Teacher
to
Rob
6.6k
Popular Question
to
Nyksubuz
▴ 20
Popular Question
to
Govardhan Anande
▴ 150
Popular Question
to
Lhl
▴ 760
Recent Replies
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ashkan
▴ 160
@atpoint the question is updated. the first command shows how the index is built.
Comment: Help! Reconstruction of locus, order contigs
by
dthorbur
★ 2.0k
Is the *de novo* annotation required for each contig? Reconstructing homologous regions in many genomes can be tricky regardless, but is es…
Comment: In one PCA plot, can I calculate the percentage of different factors that contri
by
diqixiaoyaoer
▴ 20
You can have a look on my answer. I have post it below.
Answer: In one PCA plot, can I calculate the percentage of different factors that contri
by
diqixiaoyaoer
▴ 20
Here is my script that can be used to calculate how much degree that different factors contribute to PCA variance (PC1 and PC2). # …
Comment: Details on salmon index
by
Lorenzo
• 0
Thank you so much for the beautiful explanation. Just one more curiosity. So in the first versions Salmon utilized RapMap to index and then…
Comment: What marks a De-Novo Genome assembly as FAILED?
by
dthorbur
★ 2.0k
I'm a little confused about your experimental design. Are you making 45 different assemblies? Or are all the samples from the same individu…
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ATpoint
82k
You should be stating how you constructed the index, and include the actual lines for the problematic genes from the abundance files. Note …
Comment: zero TPM for many gene including housekeeping when using kallisto
by
dariober
14k
You could try [salmon](https://combine-lab.github.io/salmon/getting_started/) to see if the problem is with kallisto or with the pseudo-ali…
Comment: solution-Transcriptome.cpp:18:Transcriptome: exiting because of *INPUT FILE* err
by
GenoMax
142k
> INPUT FILE error: could not open input file /geneInfo.tab Based on the error it appears that the input file you are providing could not …
Answer: How to handle duplicated genes in TCGA data?
by
txema.heredia
▴ 130
Having duplicate gene symbols is pretty common. Why do you need to work with gene symbols? To compare them to external datasets? Or just fo…
Comment: Merge clusters in Seurat UMAP
by
kilcdincer
▴ 10
It did not help either. But I got the answer [here][1] Thank you! [1]: https://github.com/satijalab/seurat/issues/8902
Comment: How to generate table_annovar from VCF input?
by
Pierre Lindenbaum
161k
there is no `--allsample` option in the "usage" screen you're showing. I suspect you're using an old version ?
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
by
egascon
• 0
Thank you very much again. I have learned a lot of things.
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
by
egascon
• 0
It's a microarryay experiment, not RNASeq
Comment: Sam file Header problem
by
Pierre Lindenbaum
161k
https://gatk.broadinstitute.org/hc/en-us/articles/360035890671-Read-groups
Traffic: 2173 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6