I would like to extract sequence with specific coordinate. I did the blast search and now from this output I need to extract the 100bp upstream and downstream from the subject sequence to whom query sequence matched in blastn search. (which is subject start and subject end). I have gone through the similar problems and their solution and tried them. I tried the following approach from brentp enter link description here and I have the error.
python seq_ext_coordinate.py Traceback (most recent call last): File "seq_ext_coordinate.py", line 5, in <module> EST_030516_lnr.fa = sys.argv IndexError: list index out of range
Does anybody have the idea what I did wrong?