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written 2.4 years ago by
Istvan Albert ♦♦ 80k
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Bioconductor (Bioc) 3.9
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CRISPR Genome Editing
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written 13 months ago by
Devon Ryan ♦ 89k
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Recent Votes
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Recent Replies
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C: Identify jumbled transcripts (scrambled exons) using HISAT2, stringtie, and GFFc by
genomax ♦ 65k
Are your individual reads long enough that they will span these shuffled exons and also give you ...
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C: Identify jumbled transcripts (scrambled exons) using HISAT2, stringtie, and GFFc by
wolfgang.rumpf • 0
Okay, that makes sense. But what about the downstream pipeline after minimap2? Was I right in a...
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C: Identify jumbled transcripts (scrambled exons) using HISAT2, stringtie, and GFFc by
genomax ♦ 65k
If you have PacBio data why are you using HISAT2? A proper long read aligner (that can not only a...
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C: R Version problems by
RamRS ♦ 21k
What's your R version? Also, these are just warnings and can be ignored unless you encounter prob...
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C: What percent of reads should align to a reference gene to successfully validate by
genomax ♦ 65k
Is that result (no reads aligning in a sample) supported by adequate biological replicates? If yo...
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A: What percent of reads should align to a reference gene to successfully validate by
lshepard • 210
You should follow the statistical cutoff used for your analysis along with perhaps a fold change ...
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A: Mapping CLIP-seq Regions to 5'UTR, CDS, or 3'UTR by
mb314 • 0
Following up in case someone has this question, I ended up using HOMER's annotatePeaks.pl. It was...
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C: Trouble with BLAST using Biopython by
jrj.healey ♦ 11k
Great, if you ever figure out exactly what it was, please do post up :) BTW, once a post has ans...
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C: 1000 genomes genotypes interpretation by
Pierre Lindenbaum ♦ 119k
VCF specification https://samtools.github.io/hts-specs/VCFv4.2.pdf . section 1.4.2 "genotype fields"
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C: Running Freebayes on 100+ Samples by
tpaisie • 70
Hello tpaisie! We believe that this post does not fit the main topic of this site. Fixed probl...
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C: Trouble with BLAST using Biopython by
tpaisie • 70
Hello tpaisie! We believe that this post does not fit the main topic of this site. Fixed probl...
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A: Trouble with BLAST using Biopython by
tpaisie • 70
I got the script to work finally! Some set up must have been off on my computer or with my biopy...
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