User: YaGalbi

gravatar for YaGalbi
YaGalbi1.3k
Reputation:
1,330
Status:
Trusted
Location:
Biocomputing, MRC Harwell Institute, Oxford, UK
Website:
https://github.com/roo...
Last seen:
1 day, 9 hours ago
Joined:
2 years, 2 months ago
Email:
r**********@gmail.com

Microbiology BSc Hons 2016

Bioinformatics MSc Distinction 2017

Bioinformatics Prize 2017

Posts by YaGalbi

<prev • 373 results • page 1 of 38 • next >
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Comment: A: What crosses to make to breed a homozygous individual?
... This looks like a high school homework or undergraduate home study question. What work have you done? What have you found out by yourself. Where are you getting confused? ...
written 1 day ago by YaGalbi1.3k
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Comment: C: Can i use ucsc track browser without a reference organism?
... Here are 2 MSA visualisation tools: [MSAViewer][1] and [Wasabi][2] Here are 2 R packages doing a little more: [MSA (Bioconductor)][3] and [Balcony (Cran)][4] But could we get some detail on what "features" you are trying to view/compare? [1]: http://msa.biojs.net/ [2]: http://wasabiapp.org/ ...
written 2 days ago by YaGalbi1.3k
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Comment: C: Confused about merging RNA-seq lanes/runs
... Thanks Genomax. I read the key point as "Since you are sampling from **the same library data** produced by multiple runs should have similar distribution of reads." So lans/runs don't really matter as long as they come from the same library? For clarity, I'm defining a library as a single epindorf ...
written 3 months ago by YaGalbi1.3k
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Confused about merging RNA-seq lanes/runs
... This question: ["Can we concatenate two fastq files from same sample but different runs"][1] has me confused unfortunately. Maybe I should know better by now , but rather than sweeping it under the carpet I'd rather clear up the confusion: I was under the impression that any form of replicate sh ...
lanes merge samples runs rna-seq written 3 months ago by YaGalbi1.3k • updated 3 months ago by genomax55k
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Comment: C: Can we concatenate two fastq files from same sample but different runs
... Whether a separate run or separate lane makes little difference, they are still technical replicates. They should not be merged. ...
written 3 months ago by YaGalbi1.3k
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Answer: C: Picard MarkDuplicates flag vs remove issue
... Mark duplicates is doing something other than just removing duplicates, otherwise it would just be called remove duplicates. My guess is that 17M have flag 1024, and the 3M have a different flag - but what is it? I would find a command that lets me print the 3M reads that are the difference between ...
written 3 months ago by YaGalbi1.3k
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Comment: C: Picard MarkDuplicates flag vs remove issue
... "However if I run it with REMOVE_DUPLICATES=FALSE and then use samtools to remove the 1024 flagged reads I end up with 56 million reads" " It's not that using Samtools rmdup is removing fewer reads, I have never even tried it" How do you expect an answer if you can't give clear information in the ...
written 3 months ago by YaGalbi1.3k
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Comment: C: Is the sequence quality good enough?
... Yes, I've started the pipeline for the data, should have the bams tomorrow and the vcfs on friday. thank you. ...
written 3 months ago by YaGalbi1.3k
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Comment: C: Is the sequence quality good enough?
... exactly what i was thinking - it could be better, but it really isn't that bad. ...
written 3 months ago by YaGalbi1.3k
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Comment: C: Is the sequence quality good enough?
... Edited ... thank you ...
written 3 months ago by YaGalbi1.3k

Latest awards to YaGalbi

Popular Question 16 days ago, created a question with more than 1,000 views. For Cuffdiff not performing tss, cds, splicing, promoter or CDS tests
Popular Question 23 days ago, created a question with more than 1,000 views. For Can anyone suggest a RShiny app for displaying RNA-seq results online
Popular Question 6 weeks ago, created a question with more than 1,000 views. For Meme: What is the optimal sequence length?
Popular Question 6 weeks ago, created a question with more than 1,000 views. For Meme: What is the optimal sequence length?
Popular Question 9 weeks ago, created a question with more than 1,000 views. For Meme: What is the optimal sequence length?
Scholar 3 months ago, created an answer that has been accepted. For C: Displaying genes in genome browsers
Scholar 3 months ago, created an answer that has been accepted. For C: Displaying genes in genome browsers
Scholar 3 months ago, created an answer that has been accepted. For C: Displaying genes in genome browsers
Voter 4 months ago, voted more than 100 times.
Popular Question 4 months ago, created a question with more than 1,000 views. For Rshiny - How do I check if user input exists in my data set?
Popular Question 4 months ago, created a question with more than 1,000 views. For Rshiny - How do I check if user input exists in my data set?
Popular Question 4 months ago, created a question with more than 1,000 views. For Quantile normalisation: raw rpkm or log2rpkm?
Popular Question 4 months ago, created a question with more than 1,000 views. For Rshiny - How do I check if user input exists in my data set?
Autobiographer 6 months ago, has more than 80 characters in the information field of the user's profile.
Popular Question 7 months ago, created a question with more than 1,000 views. For RNA-seq RPKM significance cut off
Commentator 8 months ago, created a comment with at least 3 up-votes. For A: wig files to bigwig using UCSC kent
Guru 8 months ago, received more than 100 upvotes.
Scholar 10 months ago, created an answer that has been accepted. For C: Displaying genes in genome browsers
Scholar 10 months ago, created an answer that has been accepted. For C: Displaying genes in genome browsers
Popular Question 10 months ago, created a question with more than 1,000 views. For RNA-seq RPKM significance cut off
Scholar 10 months ago, created an answer that has been accepted. For C: Displaying genes in genome browsers
Popular Question 15 months ago, created a question with more than 1,000 views. For RNA-seq RPKM significance cut off
Scholar 15 months ago, created an answer that has been accepted. For C: Displaying genes in genome browsers
Teacher 15 months ago, created an answer with at least 3 up-votes. For A: Looking for an annotation tool
Student 16 months ago, asked a question with at least 3 up-votes. For Is annotation of enhancers to nearest gene pointless?

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