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bcftools: error while loading shared libraries: libcrypto.so.1.0.0: cannot open shared object file: No such file or directory
bcftools libcrypto.so.1.0.0
updated 11 minutes ago by • 0 • written 14 months ago by ▴ 100
0
votes
0
replies
5
views
Different SNP at the at a position in vcf file compared to REF and ALT allele.
Genome sequencing variant calling
21 minutes ago by Abbas.M • 0
0
votes
0
replies
8
views
Is it reasonable to do "Gene Regulatory Network Inference" for each cell type and do differential GRN
GRN scRNA-seq
1 hour ago by shangguandong1996 ▴ 30
0
votes
0
replies
13
views
Expression data of diabetic patients
Diabetes
2 hours ago by shiviagarwalpharma • 0
0
votes
0
replies
39
views
How do I get separate ADT / CITE-seq fastq's from single SRA / BAM files? (originally generated from cellranger)
cellranger scRNA SRA CITE-Seq sra-tools
4 hours ago by msn ▴ 40
0
votes
0
replies
32
views
moving .txt from linux to Mac desktop
file .txt moving
4 hours ago by smithkthedale ▴ 10
2
votes
3
replies
129
views
Same results in Limma Toptable as in DESEQ2 results
DESEQ2 Limma
updated 5 hours ago by ★ 4.9k • written 13 hours ago by ▴ 40
0
votes
0
replies
34
views
DoHeatmap with GSEA gene set?
scrnaseq singlecell gsea
5 hours ago by crx6xw ▴ 10
694
votes
159
replies
95k
views
99 follow
News: The Biostar Handbook. A bioinformatics e-book for beginners.
training tutorial News handbook
updated 10 days ago by 1.0k • written 5.8 years ago by 94k
0
votes
1
reply
68
views
Job: Senior Bioinformaticians - Cancer Research
genes calling variant RNA-Seq driver research cancer
7 hours ago by David • 0
0
votes
2
replies
74
views
How to convert Ensemble Gene IDs to gene symbols or Entrez Gene IDs?
Gene Symbols mitch IDs
updated 8 hours ago by ★ 13k • written 8 hours ago by ▴ 70
0
votes
1
reply
59
views
Looking for matching names in fasta file and genbank records
biopython
updated 7 hours ago by 149k • written 8 hours ago by • 0
0
votes
3
replies
222
views
Why it is recommended to run samples from all groups together while performing DESeq although the design variables are not used when estimating the s…
DESeq2 GSEA R DEG
updated 8 hours ago by 94k • written 15 hours ago by ▴ 70
0
votes
0
replies
37
views
Performe gene co-expression analysis network on my own genes of interest scRNA-seq dataset
downstream scRNA-seq analysis
10 hours ago by Fiefn • 0
0
votes
0
replies
42
views
Do vsearch -uchime_ref needs all the reads in same direction as the reference database?
chimeras vsearch
12 hours ago by anicet.ebou ▴ 170
0
votes
0
replies
44
views
Visualization of networks inferred using GrnBoost2 on bulk mRNA expression data
python inference network visualization GrnBoost2
13 hours ago by melissachua90 ▴ 40
0
votes
1
reply
152
views
Limma Differential Expression with Duplicate Correlation
Duplicate Differential Analysis Limma Expression Correlation
updated 14 hours ago by 15k • written 1 day ago by ▴ 40
0
votes
0
replies
83
views
SMARTseq2 for non scRNAseq
dual SMART-seq2 RNA-seq
updated 11 hours ago by 65k • written 1 day ago by • 0
0
votes
0
replies
73
views
StringTie filtering settings
StringTie filtering uORF
17 hours ago by Sarah • 0
1
vote
3
replies
135
views
I want to output a file in R where I will print the longest locus.
R Programming locus lenght
updated 8 hours ago by 9.8k • written 17 hours ago by ▴ 30
0
votes
1
reply
93
views
Error/Seurat error with R/Rstudio version 4.1.1
seurat rstudio
updated 11 hours ago by 9.8k • written 18 hours ago by • 0
0
votes
0
replies
75
views
If there is a way to link two gosling html components in two <div>s.
gosling.js
18 hours ago by Weihang • 0
0
votes
1
reply
183
views
unsolved questions in RNA
isoform rna
updated 19 hours ago by 65k • written 2 days ago by • 0
1
vote
5
replies
207
views
how to create a character vector in R
vector r microarray character analysis
41 minutes ago by bioinformatics ▴ 10
0
votes
5
replies
195
views
Unable to install bioconda and bowtie
bioconda conda
updated 13 hours ago by ▴ 440 • written 19 hours ago by ▴ 30
0
votes
0
replies
83
views
Only mapping reads that match reference and are longer than a given length in Minimap2
Bioinformatics
20 hours ago by zack.saud ▴ 50
0
votes
2
replies
164
views
renaming files and including folder/directory name in it
rename loop mv
21 hours ago by arshad1292 ▴ 70
1
vote
3
replies
116
views
Ranking genes by a conservation score which includes enhancers
scrna-seq enhancers conservation
4 hours ago by angus.j.g.campbell ▴ 10
3
votes
3
replies
179
views
Check barcode collision
awk script bash
18 hours ago by bobia9193 • 0
0
votes
1
reply
103
views
Any option in bwa mem aligner for headless sam output?
sam bowtie2 bwa alignment
updated 1 day ago by 149k • written 1 day ago by ▴ 150
0
votes
0
replies
82
views
Help with metadata in qiime2
Qiime2 metabarcoding
1 day ago by crazy_martyn • 0
0
votes
7
replies
290
views
converting probes robes to gene IDs
gene probes IDs offtopic
2 hours ago by bioinformatics ▴ 10
1
vote
5
replies
180
views
How to select the variables with maximum values in each group with dplyr?
R dplyr
updated 1 day ago by 9.8k • written 1 day ago by • 0
0
votes
10
replies
425
views
microarray analysis in R
analysis message error microarray
51 minutes ago by bioinformatics ▴ 10
0
votes
0
replies
106
views
How to run Bionano Hybrid Scaffolding without root and Solve tool
snakemake bionano linux conda
1 day ago by snakelake • 0
0
votes
3
replies
223
views
microarray bioinformatic analysis in R
differentially expressed genes microarray
updated 1 day ago by • 0 • written 1 day ago by ▴ 10
0
votes
1
reply
137
views
macs callpeak q value cutoff 1 and 0.05 with different peak result
peak macs2 TF cutoff value q call
updated 20 hours ago by ▴ 440 • written 2 days ago by • 0
0
votes
2
replies
147
views
SVM: why am I getting different results?
python svm
2 days ago by lizabe ▴ 10
0
votes
1
reply
104
views
How can I get the seqlogo from meme
MEME
updated 2 days ago by ★ 20k • written 2 days ago by • 0
2
votes
3
replies
364
views
Concatenate information columns from multiple identical bed files.
merge genomics bedtools
2 days ago by nicole.zeltser • 0
0
votes
1
reply
142
views
calling peaks in ChIP-seq datasets from bedgraph files
ChIP-seq MACS2
updated 20 hours ago by ▴ 440 • written 2 days ago by ★ 1.3k
5
votes
7
replies
529
views
Limma Differential Expression contrast matrix with several variables
Expression Differential Limma
19 hours ago by Bine ▴ 40
2
votes
3
replies
5.2k
views
Any bacterial databases that contain Gram staining information?
database gram positive gram negative
updated 2 days ago by • 0 • written 6.2 years ago by • 0
0
votes
0
replies
95
views
Identifying gene expression patter for B cell malignancies
GEX Lymphoma
2 days ago by BcellPhil • 0
0
votes
1
reply
137
views
xcode license agreement
xcode conda
updated 2 days ago by 52k • written 2 days ago by • 0
0
votes
1
reply
115
views
How to pair TCGA bam files with no properly paired reads?
GDC TCGA Paired-Read BAM
updated 14 hours ago by 15k • written 2 days ago by • 0
0
votes
0
replies
247
views
Job: Repost: Bioinformatics Research Scientist-Epigenetics(USA, Memphis, TN, St. Jude Children's Research Hospital)
Bioinformatics Memphis Epigenetics Hospital
2 days ago by xubeisi ▴ 20
1
vote
2
replies
147
views
How to fix: Error: No module named HTSeq.scripts?
HTSeq
1 day ago by Chris ▴ 20
0
votes
4
replies
173
views
Help with plotting DEG-specific heatmap
Heatmap DEG
2 days ago by marina.wakid • 0
0
votes
3
replies
181
views
Pearson Correlation Single cell seurat
singlecell seurat
updated 1 day ago by 810 • written 2 days ago by ▴ 140
105,114 results • Page 1 of 2103
Recent Votes
bcftools: error while loading shared libraries: libcrypto.so.1.0.0: cannot open shared object file: No such file or directory
Answer: bcftools: error while loading shared libraries: libcrypto.so.1.0.0: cannot open
getting a consense one header .fasta file from a genic region, from .bam template
C: getting a consense one header .fasta file from a genic region, from .bam templat
C: getting a consense one header .fasta file from a genic region, from .bam templat
getting a consense one header .fasta file from a genic region, from .bam template
Comment: Ranking genes by a conservation score which includes enhancers
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Answer: bcftools: error while loading shared libraries: libcrypto.so.1.0.0: cannot open by felixm • 0
Updating `bcftools` to `1.15.1` fixed this for me. `conda install -c bioconda -c conda-forge bcftools=1.15.1`
Comment: Using QCTOOL v2 to process UK Biobank .bgen files - why so slow? by yayuan • 0
Thank you so much!! I have visited the directory and tried to find out the one suit my system, but failed. however, i got version 2.0.7 las…
Comment: microarray analysis in R by bioinformatics ▴ 10
I have the probe names listed as a column in the excel file with p values for differentially expressed genes. This is the code I used: …
Comment: how to create a character vector in R by bioinformatics ▴ 10
I have performed microarray differential expression analysis using the following commands: library("arrayQualityMetrics") library(…
Comment: converting probes robes to gene IDs by bioinformatics ▴ 10
Ok thanks, I have a list of 16384 probe names in a text file that are unique and not sequential. Is there are way of converting this column…
Comment: how to create a character vector in R by bioinformatics ▴ 10
Thanks for your reply, my probe list is a txt file and a column in excel file with my p values from differential gene expression.
Comment: How to resolve a ValueError: Multiple 'HD' Lines are not permitted when I run Ci by liyuguo • 0
The code for deletion is **samtools view -h unknown_circle.sam | sed -e '/HD/d' > test_circle.sam**
Comment: How to resolve a ValueError: Multiple 'HD' Lines are not permitted when I run Ci by liyuguo • 0
Wow, I tried to delete the "HD" line from the original Sam file and continue with the circle-map pipeline, and the error disappeared
Comment: Ranking genes by a conservation score which includes enhancers by angus.j.g.campbell ▴ 10
Thanks for the suggestion about UCell I will try this out.
Comment: Ranking genes by a conservation score which includes enhancers by angus.j.g.campbell ▴ 10
Our lab only uses publicly available data we don't do experiments ourselves so FACS is out of the question. Also the training data was col…
Answer: Same results in Limma Toptable as in DESEQ2 results by Gordon Smyth ★ 4.9k
It would be superfluous to include a SE column in the limma output because it is not needed and because it is immediately computable from t…
Comment: htseq-count: error when reading beginning of SAM/BAM file. by Chris ▴ 20
Hi @genomax, would you please explain why my output is different? samtools view ~/protocol_1/data/MT1/Tophat_Out/accepted_hits.sorted.…
Comment: Looking for matching names in fasta file and genbank records by Pierre Lindenbaum 149k
find DIR1 DIR2 -type f -name "*.gb" | grep -F -f <(grep "^>" in.fa | cut -c 2-)
Comment: paired-end reads, idxstats, and samtools and grep by Vincent Laufer ★ 2.4k
oke we are waiting. :-)
Answer: Senior Bioinformaticians - Cancer Research by David • 0
https://www.sanger.ac.uk/group/adams-group/
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