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Please use `ADD REPLY` when responding to existing comments/posts to keep threads logically organized.
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Hi Ben, thanks for your reply.
Here I copy part of the row with the information.
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Please use `-o sorted.bam` to save the sorted BAM in your command line. This is standard option with all recent versions of `samtools`.
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Just an added query, I checked the output bam file from the above using picard ValidateSamFile and its missing the read groups. Is there a …
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Thanks!
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Hum strange, and if you set `plotPCA <- DESeq2::plotPCA` before running `plotPCA(rld, intgroup=c("Diet"))`, do you still have the same erro…
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Still getting the same error-
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