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Recent Replies
Comment: hisat2 /usr/bin/env: ‘python’: No such file or directory
by
Mensur Dlakic
★
14k
You have two options, and both of them require admin privileges by you, or by someone who can make these changes for.
The first option is …
Comment: hisat2 /usr/bin/env: ‘python’: No such file or directory
by
Xiaoyun
•
0
here is my result:
which python3
/usr/bin/python3
but `hisat2-buildis` read only, I can't edit it.
Comment: hisat2 /usr/bin/env: ‘python’: No such file or directory
by
Xiaoyun
•
0
here is my result:
which python3
/usr/bin/python3
but `hisat2-build`is read only, I can't edit it.
Comment: Complex Heatmap
by
Kevin Blighe
77k
Please show the code that you're running. I used the `factor()` approach just now in my own data, and it does work.
Comment: hisat2 /usr/bin/env: ‘python’: No such file or directory
by
GenoMax
107k
Rather surprising that you have no system `python` installed. As @mensur already said below you will need to install `python`.
Comment: Complex Heatmap
by
arsa
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10
Hi Kevin,
Thanks for the reply. I could change the cluster order. Can you help me how can I reannotate the clusters now. I need to reanno…
Answer: Can recombination take place in bacterial protein coding gene?
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Rui
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40
Thank you Dr.Albert. By reading a bit further, it seems part of the reason why removing the recombinant sequences is not advisable is becau…
Answer: Complex Heatmap
by
Kevin Blighe
77k
Hi,
You need:
...,
column_split = factor(cluster_anno, levels = c(0,1,2,3,4,5,6)),
...,
Failing that, take a look at these t…
Answer: Stool miRNA - low alignment rate
by
biobiu
▴
120
You enriched your library with small-RNA? If so, I would check that reads were preprocessed well, miRNA are short so reads will usually con…
Comment: hisat2 /usr/bin/env: ‘python’: No such file or directory
by
Xiaoyun
•
0
but after I run this command
which python
the output is
/usr/bin/which: no python in (/home/yun/Downloads/hisat2-2.2.0:/home/…
Comment: hisat2 /usr/bin/env: ‘python’: No such file or directory
by
Xiaoyun
•
0
/usr/bin/which: no python in (/home/yun/Downloads/hisat2-2.2.0:/home/yun/Downloads/Trimmomatic-0.39:/home/yun/Downloads/FastQC:/home/yu…
Comment: Stool miRNA - low alignment rate
by
Jeremy Leipzig
20k
are you aligning against the actual genome or mirbase hairpins? i think mirdeep is a wrapper that does either.
Comment: Convert transcriptomic bam to genomic bam
by
Ram
34k
You could email the RSEM developer and check with them on what you could do to make the script work better for you.
Comment: PEPTIDES IN PRIDE
by
GenoMax
107k
You may have already done this but can you tell us if you have looked at [**this help page**][1]? It has a section on [**searching data in …
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