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Recent Replies
Answer: Meaning of read color on IGV
by
Pierre Lindenbaum
145k
Coloring of Mapped Paired Reads in https://software.broadinstitute.org/software/igv/AlignmentData
https://software.broadinstitute.org/sof…
Comment: Bulk RNA seq: all p adjusted are the same
by
nils.simon.biology
•
0
Hi,
Yes they did QC I went through the report they provided and the single steps with a bioinformatician from our university (only availabl…
Comment: Trimmomatic trimming principle
by
Arrate
•
0
So you meant that if after the adapter sequence, it is detected again a match with the technical sequence, the read is supposed to be a ada…
Comment: A check over my code using Limma DE, starting from normalized data
by
cpad0112
20k
RNAseq data is mostly modeled after NB distribution, for biological replicates. Since data is normalized (TPM) and subsequently log transfo…
Comment: arrange samples in same order in rows and column in R
by
Basti
▴
360
Normally yes, you can check that your design matrix second column refer to your logeGFR variable.
For the second question, I do not know wh…
Comment: A check over my code using Limma DE, starting from normalized data
by
ATpoint
61k
Don't, it is not going to be normal anyway. Read this here for code suggestions -- https://support.bioconductor.org/p/125144/#125156 -- TP…
Comment: Is there a way to expand RAM or CPU?
by
ATpoint
61k
See also my comment here. https://www.biostars.org/p/9524489/#9524514
Comment: How to install PEERTOOLS in a cluster?
by
fernandogs97
•
0
Thank you for your help, just if anyone see this I finally decided to use the R version of this program following the instructions describe…
Comment: Is there a way to expand RAM or CPU?
by
Joe
20k
All motherboards support expandable RAM and usually a family of CPUs. If memory is the issue it is unlikely to be a CPU issue.
Whether or …
Comment: Is there a way to expand RAM or CPU?
by
Joe
20k
Co-lab is an online coding environment where the code is executed on the fly by virtual machines (usually). This means it doesn't run on yo…
Comment: A check over my code using Limma DE, starting from normalized data
by
JACKY
▴
10
I don't think any data of this type (RNA-seq) is normally distributed.. or is it ?
Comment: Python program to convert UniProt to PDB
by
Joe
20k
This isn't a question, and Biostars is not a coding service. Please put more effort in.
Comment: Mouse miRNA mapping
by
AellSidious
•
0
I will try with bowtie2 then. Thank you for the suggestion.
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