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Recent Replies
Comment: FASTA of translated amino acid sequences in their six reading frames, which one
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Luis999
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Oooh, thank you thank you thank you, I'm already reading the documentation for the programs you recommended. Borf looks promising. Another …
Comment: FASTA of translated amino acid sequences in their six reading frames, which one
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Again, I didn't think I would get answers so fast, for real, thank you very much!!! Yes, I was referring to the longest sequence, after sea…
Comment: FASTA of translated amino acid sequences in their six reading frames, which one
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Wow, I didn't think I would get answers so fast, thank you very much !!! By "Optimal" I meant the longest sequence
Comment: Help with Volcano plot
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![enter image description here][1]Tried. However, the plot does not look well volcanic.
[1]: /media/images/bdc16f6e-7265-4b25-b75b-8991…
Comment: Help with Volcano plot
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Please change the threshold to 0.0. This will mean, however, that `FindMarkers()` takes longer to complete.
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but how I will set, I have to create heat map on entire data
Comment: Help with Volcano plot
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Hi,
Yes, possibly due to As.response was generated by using FindMarker() with 0.25 as a cutoff.
Please let me know if there is a better way…
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Generally, we recommend that featureCounts be run with default settings, which does not include --primary. Just follow the documentation. T…
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what code did you use to generate `As.response`? It looks like genes with a |log2 fold change| < 0.25 were potentially filtered out of the …
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Hi, you could check a software names KaKs Calculator (DOI: 10.1016/S1672-0229(07)60007-2) not KaKs Calculator 2.0 probably, i am using it n…
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> It is assumed that similar sequences perform similar functions. Is the converse also true?
`Fairly similar` sequences are likely to perf…
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Thanks for your answer.
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This will be a useful read: https://www.ncbi.nlm.nih.gov/books/NBK20255/
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Without much description on what your python script looks like, I can't say what is going wrong. Have you considered using [PGDSpider][1]? …
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