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119,213 results • Page
1 of 2385
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Votes
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0
votes
2
replies
35
views
Highly skewed distribution of differentially expressed (DE) genes
R
RNAseq
Deseq2
updated 14 minutes ago by
LChart
4.3k • written 1 hour ago by
Maria
• 0
1
vote
5
replies
52
views
Differential expression analysis between groups with varying cell proportions
scRNA-Seq
Seurat
Pseudobulk
14 minutes ago by
AB
▴ 360
0
votes
1
reply
29
views
QIIME2 artifact in galaxy
help
updated 1 hour ago by
GenoMax
146k • written 2 hours ago by
mbyec18
• 0
0
votes
2
replies
59
views
RNA-seq heatmaps: effective length, RPKM vs. CPM
edgeR
RNA-seq
updated 2 hours ago by
ATpoint
85k • written 3 hours ago by
bioinfo2345
▴ 40
0
votes
3
replies
66
views
The definition of a gene
gene
lncrna
transcript
updated 1 hour ago by
Ram
44k • written 3 hours ago by
zizigolu
★ 4.3k
4
votes
7
replies
144
views
Extracting single chromosome from WGS cram/bam files before downloading
VNTR
1KG
HGDP
SGDP
WGS
updated 3 hours ago by
Pierre Lindenbaum
164k • written 5 hours ago by
berndmann
• 0
0
votes
0
replies
42
views
Performing sex-stratified analysis using regenie
regenie
updated 1 hour ago by
Ram
44k • written 7 hours ago by
derycine
• 0
0
votes
0
replies
56
views
HOMER annotateInteractions.pl error
annotateInteractions.pl
HOMER
11 hours ago by
anushkachaturvedi099
• 0
819
votes
169
replies
157k
views
110 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 4 weeks ago by
Biostar
3.0k • written 7.9 years ago by
Istvan Albert
101k
2
votes
1
reply
93
views
variant calling through gatk
gatk
vcf
variant-calling
updated 1 hour ago by
Ram
44k • written 11 hours ago by
ashaneev07
▴ 30
2
votes
1
reply
105
views
Downloading Raw Reads
reads
RNA
updated 1 hour ago by
Ram
44k • written 12 hours ago by
Jamie
• 0
1
vote
1
reply
88
views
Good Dotplots for homogenous data (Seurat)
seurat
dotplot
updated 9 hours ago by
yura.grabovska
▴ 570 • written 13 hours ago by
carolofharvest
▴ 40
1
vote
1
reply
143
views
1000 genome dataset download
ADMIXTURE
1000genome
iAdmix
fastNGSadmix
plink
updated 14 hours ago by
chrchang523
11k • written 1 day ago by
Soohyun
• 0
0
votes
2
replies
128
views
Immunedeconv CIBERSORT
p-value
Immunedeconv
CIBERSORT
14 hours ago by
dy001
• 0
2
votes
6
replies
166
views
errors in installing picard
picard
updated just now by
GenoMax
146k • written 19 hours ago by
Meghan.T
• 0
1
vote
4
replies
198
views
Bulk RNA seq PCA plot issues
RNA-seq
PCA
updated 17 hours ago by
Ram
44k • written 22 hours ago by
karlensberg
• 0
0
votes
0
replies
69
views
News:
Upcoming November Courses – Enhance Your Skills in Bioinformatics and Data Analysis
Population-Genomics
scRNA-seq
RNA-seq
Metagenomics
Genome-Assembly
22 hours ago by
carlopecoraro2
★ 2.6k
0
votes
0
replies
76
views
SVA with intercept only
SVA
batch-correction
updated 22 hours ago by
Ram
44k • written 23 hours ago by
IM
• 0
4
votes
2
replies
143
views
Problem in calling peak by MACS3
MACS3
updated 1 day ago by
ATpoint
85k • written 1 day ago by
daffodil
▴ 10
0
votes
1
reply
887
views
Using TCGABioLinks to query multiple cancer studies
RNA-Seq
R
TCGA
updated 1 day ago by
GenoMax
146k • written 3.7 years ago by
ivykosater
• 0
0
votes
1
reply
231
views
News:
Online Workshop - Bioinformatics Pipeline Development with Nextflow (November 13-15, 2024)
Workshop
Nextflow
RNA-Seq
1 day ago by
ecSeq Bioinformatics
• 0
0
votes
1
reply
114
views
Merging BAM files after alignment or restarting the workflow?
BAM
alignment
updated 1 day ago by
GenoMax
146k • written 1 day ago by
stanmih2
• 0
1
vote
1
reply
125
views
Raw reads or raw counts for meta-analysis
meta-analysis
rna-seq
HTSEQ
STAR
updated 22 hours ago by
Ram
44k • written 1 day ago by
a.stef.44
▴ 10
1
vote
6
replies
320
views
Can someone explain to me in relatively simple language how PCR primer, reverse primer, and probe sequences are generated?
reverse
probe
pcr
primer
12 hours ago by
Ryan
• 0
1
vote
3
replies
177
views
How to determine whether it is 10x data
GEO
10x
SRA
updated 22 hours ago by
Ram
44k • written 1 day ago by
king
• 0
2
votes
4
replies
235
views
Tool:
fastplong: ultrafast preprocessing and quality control for long reads
long-reads
updated 21 hours ago by
GenoMax
146k • written 1 day ago by
chen
★ 2.5k
2
votes
8
replies
275
views
GEOquery issue
database
GEO
GEOquery
21 hours ago by
Jaber
▴ 30
0
votes
0
replies
85
views
Need help with providing RNA Seq data to run braker.pl in ETP mode.
augustus
gene-prediction
rna-seq
braker
genemark
1 day ago by
Vijith
▴ 60
0
votes
2
replies
136
views
Can the CFD commercial code simulate multicellular flow in a tall cavity using the second-order scheme?
multicellular-flow
updated 22 hours ago by
GenoMax
146k • written 1 day ago by
uthai
• 0
0
votes
1
reply
126
views
How to filter a reference genome for single copy genes?
busco
genes
single
copy
genome
updated 1 day ago by
Pierre Lindenbaum
164k • written 1 day ago by
Armin
• 0
0
votes
0
replies
116
views
Job:
Full Time Bioinformatics Analyst: University of Connecticut: Computational Biology Core
bioinformatician
1 day ago by
Jlw
▴ 190
0
votes
0
replies
74
views
How to obtain level 2 GO term from go_id?
R
GO-term
1 day ago by
Chenfeng
• 0
2
votes
0
replies
95
views
Blog:
How Seurat PCA projection, KNN and MNN for label transfer work
R
RNA-seq
scRNA-seq
PCA
updated 22 hours ago by
Ram
44k • written 1 day ago by
Ming Tommy Tang
★ 4.4k
0
votes
0
replies
80
views
News:
RNAseq in non-model organisms (online course)
Bioconductor
Pacbio
RNAseq
1 day ago by
carlopecoraro2
★ 2.6k
1
vote
3
replies
186
views
WGS sequence composition bias at read start
WGS
updated 1 day ago by
GenoMax
146k • written 1 day ago by
Mega
• 0
0
votes
2
replies
225
views
Does BWA-MEM 2 account for overlapping reads within the paired end data?
mapping
calling
variant
reads
2 days ago by
ayeraselvan
▴ 10
0
votes
0
replies
114
views
Forward strand alignment
QC
GWAS
strand
alignment
8 hours ago by
JJ
• 0
3
votes
2
replies
222
views
Is there an easy way to scrape a whole bunch of NCBI RefSeq FASTA from NCBI?
FASTA
Genome
NCBI
updated 3 days ago by
shenwei356
8.6k • written 3 days ago by
Mark
▴ 10
0
votes
0
replies
113
views
Trouble with CGenFF and Ligand Topology in GROMACS
CGneFF
Protein-ligand
Gromacs
MD
simulation
3 days ago by
Mostafa
• 0
106
votes
109
replies
52k
views
29 follow
Tutorial:
Produce PCA bi-plot for 1000 Genomes Phase III - Version 2
1000genomes
PLINK
PCA
updated 2 days ago by
LChart
4.3k • written 6.1 years ago by
Kevin Blighe
88k
0
votes
3
replies
219
views
GTF merging with gffcompare
gtf
merging
annotation
RNA-Long
reads
transcript
updated 1 day ago by
Carlo Yague
8.9k • written 3 days ago by
Mai.Nabil
• 0
0
votes
2
replies
214
views
How methylation is annotated, and what is the number behind the cg.... annotation
Methylation
annotation
3 days ago by
Peter
• 0
0
votes
1
reply
164
views
Rnbeads
Rnbeads
methylation
DNA
R
updated 3 days ago by
Ram
44k • written 3 days ago by
AJ
• 0
0
votes
5
replies
345
views
Overlapping reads, how do we counter them while variant calling, haplotype calling, does it affect mapping?
reads
updated 1 day ago by
Jeremy Leipzig
22k • written 3 days ago by
ayeraselvan
▴ 10
1
vote
1
reply
297
views
(Nextflow) Having trouble with a generic process to stitch together files that were split and then processed
nextflow
updated 3 days ago by
Pierre Lindenbaum
164k • written 3 days ago by
sodiumnitrate
▴ 20
1
vote
4
replies
315
views
Gene set enrichment analysis gives a lot of "significant pathways"
KEGG
GESA
Hallmarks
updated 9 hours ago by
yura.grabovska
▴ 570 • written 4 days ago by
Bine
▴ 70
4
votes
2
replies
197
views
How can I check if a variant affects a TFBS?
variant
TFBS
3 days ago by
Tania
▴ 180
0
votes
0
replies
106
views
How to estimate haplotype number from pooled dna sequences from blood samples
sequences
Haplotype
pooled
4 days ago by
Arline
• 0
0
votes
0
replies
106
views
Deduplication when performing copy number analysis
CNV
copy-number
updated 3 days ago by
Ram
44k • written 4 days ago by
Kenji YT
• 0
0
votes
2
replies
196
views
intersection of vcf files with bcf isec - no header
isec
bcftools
vcf
1 day ago by
jasminestaples1
• 0
119,213 results • Page
1 of 2385
Recent Votes
Comment: Differential expression analysis between groups with varying cell proportions
Comment: variant calling through gatk
Comment: CUT&Tag data processing: peak caller choice and typical peak size profile
Comment: Extracting single chromosome from WGS cram/bam files before downloading
Comment: errors in installing picard
Comment: errors in installing picard
A: GATK; Variant calling RNA-Seq best practices: bam processing steps
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Recent Replies
Comment: errors in installing picard
by
GenoMax
146k
Not delete this post. Pierre was asking you to post the comment above with the error as a new question since it is not related to your orig…
Comment: How to convert raw Nanopore R9 fast5 files to fastq files ?
by
GenoMax
146k
Main point is you want to make sure you use the right pore version. That seems to automagically happen if you have internet access.
Comment: Highly skewed distribution of differentially expressed (DE) genes
by
LChart
4.3k
> Specifically, 514 genes are down-regulated (log2 Fold Change < 0), while 1152 genes are up-regulated (log2 Fold Change > 0). This uneven…
Comment: Differential expression analysis between groups with varying cell proportions
by
AB
▴ 360
Thank you. Is there a specific test in FindMarkers that might be better suited for this ? I've always run the default wilcoxon. Maybe MAST …
Comment: errors in installing picard
by
Meghan.T
• 0
Did you mean to delete the post? I can't find a close option unfortunately.
Comment: How to convert raw Nanopore R9 fast5 files to fastq files ?
by
lieven.sterck
15k
interesting point though: would you assume that most (all?) always use the latest most recent model? if so then indeed the system where you…
Comment: How to convert raw Nanopore R9 fast5 files to fastq files ?
by
lieven.sterck
15k
that indeed works as well but this is in the case where you (for some reason) would like to use a specific model (and/or version of it)
Comment: How to convert raw Nanopore R9 fast5 files to fastq files ?
by
GenoMax
146k
If you have access to internet then `dorado` will automatically download the correct model. You only need to specify the level of calling a…
Comment: How to convert raw Nanopore R9 fast5 files to fastq files ?
by
lieven.sterck
15k
Would that have changed lately? I was chatting with one of the developers of dorado recently ( in the frame of a bug report) and he told …
Comment: CUT&Tag data processing: peak caller choice and typical peak size profile
by
jared.andrews07
★ 17k
It also has disturbingly different profiles than ChIP-seq for the same TFs at times, which we aren't real sure how to interpret. Even profi…
Comment: Highly skewed distribution of differentially expressed (DE) genes
by
ATpoint
85k
Please show `plotMA()`. Anyway, a 2:1 ratio is not really concerning, there is no guarantee DE profiles are symmetrical.
Comment: gdc-client command line error
by
ATpoint
85k
That means it is not in `PATH`. Please google to learn what PATH is and how to add binaries to it.
Comment: Differential expression analysis between groups with varying cell proportions
by
ATpoint
85k
You can always subsample.
Comment: QIIME2 artifact in galaxy
by
GenoMax
146k
Please post this over at Galaxy help forum for specific help: https://help.galaxyproject.org/
Comment: The definition of a gene
by
GenoMax
146k
These are genes located in that region: http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&lastVirtModeType=default&lastVirtModeExtraState=&vi…
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