Moderator: swbarnes2

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swbarnes26.0k
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Posts by swbarnes2

<prev • 716 results • page 1 of 72 • next >
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Answer: A: scRNAseq: Split FASTQ into cell specific FASTQ Files
... You could split on the CB tag, but coverage in single cell datasets is so poor, you aren't going to find high quality variants. ...
written 2 days ago by swbarnes26.0k
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Answer: A: small wheat samples fastq or fatq.gz for testing pipeline
... Why can't you just take the first 4000 lines of your fastq? ...
written 2 days ago by swbarnes26.0k
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Answer: A: Error installing bcl2fastq 2.20 in make step - Ubuntu 18.04
... FWIW, my IT guy couldn't install bcl2fastq like this either. He used the rpm. ...
written 4 days ago by swbarnes26.0k
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Answer: A: BWA index getting killed
... Why are you trying to index a fastq file? You are supposed to index your reference file, not your read file. Your reference file should be fasta format. ...
written 4 days ago by swbarnes26.0k
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Comment: C: Setting Up Model in DeSeq2
... Does group associate with PC3 or PC4? Because group looks like it's not affecting expression much, if at all, so comparing between groups will show you little or nothing. Does PC1 correlate with batch? ...
written 7 days ago by swbarnes26.0k
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Comment: C: Error while importing data into Qiime2
... qiime2 is clearly looking for reads file that end in .gz. So why don't you zip yours (how did they get unzipped in the first place?) and try again? ...
written 8 days ago by swbarnes26.0k
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Comment: C: What kind of unit of RNA-seq should be used in machine learning models?
... In a library prep that is biased towards one end of the transcript, transcript length won't affect the number of read counts you get, so correcting for it would be wrong. ...
written 8 days ago by swbarnes26.0k
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Answer: A: Error while importing data into Qiime2
... Okay, so it looks like your fastq names don't match the regex that the import function expects. but if you choose not to tell us what your file names are, we can't possibly help you there. ...
written 8 days ago by swbarnes26.0k
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Comment: C: Setting Up Model in DeSeq2
... Sure, large amounts of noise will make it hard for the software to conclude that the difference in means between groups is significant. Your images do not work for me. ...
written 8 days ago by swbarnes26.0k
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Comment: C: Setting Up Model in DeSeq2
... If that's what your data is, that's what your data is. ...
written 8 days ago by swbarnes26.0k

Latest awards to swbarnes2

Scholar 8 days ago, created an answer that has been accepted. For A: Problem with FPKM values
Teacher 5 weeks ago, created an answer with at least 3 up-votes. For A: Exon-Seq Mutation Detection
Teacher 5 weeks ago, created an answer with at least 3 up-votes. For A: Calculating Rpkm For Rna-Seq Data Including Several Samples Each Condition
Scholar 6 weeks ago, created an answer that has been accepted. For A: Problem with FPKM values
Scholar 8 weeks ago, created an answer that has been accepted. For A: Problem with FPKM values
Teacher 8 weeks ago, created an answer with at least 3 up-votes. For A: Calculating Rpkm For Rna-Seq Data Including Several Samples Each Condition
Scholar 9 weeks ago, created an answer that has been accepted. For A: Problem with FPKM values
Scholar 9 weeks ago, created an answer that has been accepted. For A: Bam to nucleotide frequencies
Teacher 9 weeks ago, created an answer with at least 3 up-votes. For A: Calculating Rpkm For Rna-Seq Data Including Several Samples Each Condition
Popular Question 11 weeks ago, created a question with more than 1,000 views. For DESeq2 multi-factor design
Teacher 3 months ago, created an answer with at least 3 up-votes. For A: Calculating Rpkm For Rna-Seq Data Including Several Samples Each Condition
Commentator 3 months ago, created a comment with at least 3 up-votes. For C: how to identify a sequencing sample's gender
Scholar 3 months ago, created an answer that has been accepted. For A: Problem with FPKM values
Commentator 3 months ago, created a comment with at least 3 up-votes. For C: how to identify a sequencing sample's gender
Scholar 3 months ago, created an answer that has been accepted. For A: Problem with FPKM values
Teacher 3 months ago, created an answer with at least 3 up-votes. For A: Calculating Rpkm For Rna-Seq Data Including Several Samples Each Condition
Scholar 3 months ago, created an answer that has been accepted. For A: Problem with FPKM values
Scholar 3 months ago, created an answer that has been accepted. For A: Problem with FPKM values
Scholar 3 months ago, created an answer that has been accepted. For A: Problem with FPKM values
Teacher 3 months ago, created an answer with at least 3 up-votes. For A: Calculating Rpkm For Rna-Seq Data Including Several Samples Each Condition
Teacher 4 months ago, created an answer with at least 3 up-votes. For A: Calculating Rpkm For Rna-Seq Data Including Several Samples Each Condition
Scholar 4 months ago, created an answer that has been accepted. For A: Problem with FPKM values
Scholar 4 months ago, created an answer that has been accepted. For A: Problem with FPKM values
Commentator 4 months ago, created a comment with at least 3 up-votes. For C: how to identify a sequencing sample's gender
Teacher 7 months ago, created an answer with at least 3 up-votes. For A: Calculating Rpkm For Rna-Seq Data Including Several Samples Each Condition

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