User: chen

gravatar for chen
chen2.0k
Reputation:
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OpenGene
Website:
https://github.com/Ope...
Last seen:
3 days, 14 hours ago
Joined:
4 years, 10 months ago
Email:
c***@haplox.com

Libraries and tools for NGS data analysis and bioinformatics:

https://github.com/OpenGene

Posts by chen

<prev • 219 results • page 1 of 22 • next >
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Answer: A: Repost: Quality of RNA Seq Data
... Is it paired-end data or single-end data? Probably you can try [fastp][1] for quality pruning. [1]: https://github.com/OpenGene/fastp ...
written 12 days ago by chen2.0k
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Tutorial: A very simple way to detect viruses and microorganisms from mNGS FASTQ data using fastv
... `fastv` is an ultra-fast tool for identification of SARS-CoV-2 and other microbes from sequencing data. It detects microbial sequences from FASTQ data, generates JSON reports and visualizes the result in HTML reports. This tool can be used to detect viral infectious diseases, like COVID-19. This too ...
viruses covid-19 sars-cov-2 tutorial fastv written 14 days ago by chen2.0k
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Tutorial: introduce a tool for fast identification of SARS-CoV-2 and other microbes from sequencing data
... `NEW: fastv 0.6.0 released, which supports scanning a KMER collection file that contains KMERs of many microorganisms.` fastv is an ultra-fast tool for identification of SARS-CoV-2 and other microbes from sequencing data. It detects microbial sequences from FASTQ data, generates JSON reports and vi ...
covid-19 fastv tutorial sars-cov-2 written 9 weeks ago by chen2.0k
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Comment: C: Do you need a deduplication tool for FASTQ data in fastp?
... Thanks for your advice. ...
written 12 months ago by chen2.0k
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Comment: C: Do you need a deduplication tool for FASTQ data in fastp?
... Thanks, I will consider your suggestion. For deduplication, I think I can control the RAM usage to be less than 16G for processing even 1Tbp Illumina PE data. ...
written 12 months ago by chen2.0k
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Forum: Do you need a deduplication tool for FASTQ data in fastp?
... Hi, I am the author of [fastp][1], a tool to provide ultra-fast all-in-one FASTQ preprocessing functions. This tool has received 500+ stars in github (https://github.com/OpenGene/fastp), and has been cited for 40+ times since its [paper][2] published in Bioinformatics about 8 months ago. Now I am ...
forum fastp deduplication written 12 months ago by chen2.0k • updated 4 months ago by manekineko130
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Answer: A: Find illumina adaptors
... Normally they are Illumina TruSeq adapters. If you don't know it, use [fastp][1] to detect the adapters automatically. See https://github.com/OpenGene/fastp#adapters for details. Remind to enable `--detect_adapter_for_pe` option if your nextseq 500 data is paired-end. [1]: https://github.com/ ...
written 12 months ago by chen2.0k
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Answer: A: How to blast a paired end fastq file?
... You can use [fastp][1] to merge it first, then blast the merged reads See the intro here: https://github.com/OpenGene/fastp#merge-paired-end-reads [1]: https://github.com/OpenGene/fastp ...
written 12 months ago by chen2.0k
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Comment: C: help in Language
... If you only want one language, then go with Python first. ...
written 13 months ago by chen2.0k
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Comment: C: very confused with GC bias
... Some sequencers, like Illumina NovaSeq may have polyG in end of reads, which may affect GC curve. Use fastp to trim polyG and check the post-filtering data. ...
written 13 months ago by chen2.0k

Latest awards to chen

Popular Question 9 days ago, created a question with more than 1,000 views. For MutScan: Detect important mutations by scanning FastQ files directly
Popular Question 6 weeks ago, created a question with more than 1,000 views. For MutScan: Detect important mutations by scanning FastQ files directly
Appreciated 8 weeks ago, created a post with more than 5 votes. For A: Good Habit for Bioinformatics Analyst or Scientist
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Popular Question 10 months ago, created a question with more than 1,000 views. For Why blast result gives Homo sapiens BAC clone
Student 10 months ago, asked a question with at least 3 up-votes. For Is there any C API documentation for HTSLIB?
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Popular Question 12 months ago, created a question with more than 1,000 views. For Why blast result gives Homo sapiens BAC clone
Teacher 13 months ago, created an answer with at least 3 up-votes. For A: How to add read depth to gene of my interest
Popular Question 13 months ago, created a question with more than 1,000 views. For Why blast result gives Homo sapiens BAC clone
Teacher 15 months ago, created an answer with at least 3 up-votes. For A: How to add read depth to gene of my interest
Appreciated 16 months ago, created a post with more than 5 votes. For A: Good Habit for Bioinformatics Analyst or Scientist
Popular Question 19 months ago, created a question with more than 1,000 views. For Why blast result gives Homo sapiens BAC clone
Popular Question 21 months ago, created a question with more than 1,000 views. For Why blast result gives Homo sapiens BAC clone
Popular Question 21 months ago, created a question with more than 1,000 views. For Is there any C API documentation for HTSLIB?
Popular Question 21 months ago, created a question with more than 1,000 views. For Why blast result gives Homo sapiens BAC clone
Popular Question 22 months ago, created a question with more than 1,000 views. For Why blast result gives Homo sapiens BAC clone
Appreciated 23 months ago, created a post with more than 5 votes. For A: Good Habit for Bioinformatics Analyst or Scientist
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Popular Question 2.2 years ago, created a question with more than 1,000 views. For Why blast result gives Homo sapiens BAC clone
Teacher 2.3 years ago, created an answer with at least 3 up-votes. For A: Programming language for Computational Biology
Appreciated 2.3 years ago, created a post with more than 5 votes. For A: Good Habit for Bioinformatics Analyst or Scientist
Popular Question 2.4 years ago, created a question with more than 1,000 views. For Why blast result gives Homo sapiens BAC clone

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