Moderator: ATpoint

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ATpoint19k
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I hate Western blots.

Posts by ATpoint

<prev • 2,667 results • page 1 of 267 • next >
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Comment: C: BWA mapping parameters for DNA capture sequencing protocol
... You have no control over what kinds of off-targets you capturing array binds. Of course you design it to minimize off-targets but this is not a perfect process. Therefore, you always align to the entire genome. ...
written 2 hours ago by ATpoint19k
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Comment: C: Why samtools depth error
... There you already have your answer. The `chr` prefix must be removed. Please show how you removed it in the first place (command line). ...
written 3 hours ago by ATpoint19k
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Comment: C: DESeq2 nested effect with 5 variables
... For these specific questions on `DESeq2` I suggest you post this over at https://support.bioconductor.org/ to get expertise from the developer right away. ...
written 3 hours ago by ATpoint19k
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Comment: C: BWA mapping parameters for DNA capture sequencing protocol
... Whole genome, that filter for reads mapping to the genes you captured. Please use the search fucntion: https://www.biostars.org/p/787/ ...
written 4 hours ago by ATpoint19k
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Comment: C: mRNA positions from vcf
... What is unclear? The page summarizing LOC729737 and there you see that based on Ensembl automatic analysis pipeline there is some yet not verified evidence for a protein-coding gene. ...
written 4 hours ago by ATpoint19k
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Comment: C: Placing SNPs within updated genome assembly
... Is this a common genome, such as human/mouse for which liftOver chain files exist? https://software.broadinstitute.org/gatk/documentation/tooldocs/4.0.0.0/picard_vcf_LiftoverVcf.php ...
written 5 hours ago by ATpoint19k
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Comment: C: mRNA positions from vcf
... See [here][1]: ID: C9J4L2_HUMAN DESCRIPTION: SubName: Full=Uncharacterized protein; CAUTION: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. [1]: https://genome.ucsc.edu/cgi-bin/hgGene?hgg_gene=uc0 ...
written 5 hours ago by ATpoint19k
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Comment: C: Unable to install XLconnect package in R studio version 3.2.1
... Please ask this over at StackExchange as this is not bioinformatics-related. ...
written 5 hours ago by ATpoint19k
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Comment: C: How to Analyze Overlapping Peaks using ChIP-Seq Data
... BEDtools intersect ...
written 6 hours ago by ATpoint19k
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Comment: C: Unable to generate VCF using bcftools
... It would be a good start to show your command lines, which is good practice when asking questions, see https://www.biostars.org/p/357878/ ...
written 7 hours ago by ATpoint19k

Latest awards to ATpoint

Teacher 4 days ago, created an answer with at least 3 up-votes. For C: Does BWA mem work with uBAM file?
Teacher 4 days ago, created an answer with at least 3 up-votes. For C: Does BWA mem work with uBAM file?
Scholar 4 days ago, created an answer that has been accepted. For C: Does BWA mem work with uBAM file?
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Appreciated 13 days ago, created a post with more than 5 votes. For A: is fortran still used in bioinformatics?
Appreciated 14 days ago, created a post with more than 5 votes. For A: is fortran still used in bioinformatics?
Teacher 14 days ago, created an answer with at least 3 up-votes. For C: Does BWA mem work with uBAM file?
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Scholar 21 days ago, created an answer that has been accepted. For C: Does BWA mem work with uBAM file?

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