blastx strand in output file

Question

[Whoknows]

Hi

I have run blastx with outfmt 6 option on my data, but I could not find strant information in output file,

Should I run in it in different way?

Thanks.

Answer 1

[Tonor]

You need to use the start and end co-ordinates basically. If start > end, then it is -ve, and if end > start then its +ve:

http://www.drive5.com/usearch/manual/blast6out.html

But you can customise tabular formats to give extra details, see here (e.g. sstrand means Subject Strand):

Blast - Formatting Output