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6.4 years ago
dllopezr
▴
120
Hi everyone
I am working with paired-end data in Trimmomatic. This software asks for Forward and Reverse reads and outputs four files: Paired Forward, Unpaired Forward, Paired Reverse and Unpaired Reverse.
¿What is the difference between the Paired and Unpaired files? ¿Which of them I must use for the next analysis?
Thanks, Carlo. So, in the case when there is not reads discarded ¿There is no difference between paired and unpaired reads?
When there is no reads discarded, all the reads should remain paired and the unpaired files should be empty.