Seqmule Error - Failed to find vcf stats
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Entering edit mode
5.6 years ago

Hi,

I keep getting this issue in seqmule:

ERROR: Failed to find vcf-stats.
Please use 'cd /home/CLSTR/user/software/seqmule/bin/secondary/../..' and then './Build installexes'

Progams in your PATH are NOT used by SeqMule.

at /home/CLSTR/user/software/seqmule/bin/secondary/stats line 1261.

----------ERROR----------
[ => SeqMule Execution Status: step 29 FAILED at Fri Feb  7 22:28:54 CST 2020, Extract variants in custom regions]
ERROR: command failed
/home/CLSTR/user/software/seqmule/bin/secondary/../../bin/secondary/worker /home/CLSTR/user/data/sample/seqmule.02092020.57459.logs 29 "/home/CLSTR/user/software/seqmule/bin/secondary/../../bin/seqmule stats -tmpdir /tmp  -capture /home/CLSTR/user2/softwares/seqmule/database/hg19/hg19_Exon_V4.bed  -vcf Sample_result/Sample_bwamem.merge.realn.recal.0_gatk_hc.vcf -p Sample_result/Sample_bwamem.merge.realn.recal.0_gatk_hc"

!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
After fixing the problem, please execute 'cd /home/CLSTR/user/data/sample’ and 'seqmule run Sample.script' to resume analysis.
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

*This is what the code in seqmule around line 1261 looks like (line 1261 is in bold):*

sub getExe

{

for my $exename(@_)

{

if ($loc{$exename})

{

 return $loc{$exename};

} else

{

**if(my $exepath=&SeqMule::Utils::search($install_dir,$exename))**

  {

$loc{$exename}=$exepath;

return $exepath;

}

}

}

 die "ERROR: Failed to find @_\n";

Please help me understand what this error means and how I can resolve it. Thank you!

seqmule • 722 views
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