Meaning of plus symbol "+" in bcl2fastq reported barcodes
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3.5 years ago

bcl2fastq v.2.20 is reporting this...

Barcode collision for barcodes: XXXXXXXX+XXXXXXXX, XXXXXXXX+XXXXXXXX

(ATCG sequences here not Xs)

I can prevent the error by changing the number of barcode mismatches (currently set at 2), but my question is what does the plus symbol "+" separator represent here? Thanks

bcl2fastq demultiplexing sequencing illumina • 685 views
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Entering edit mode
3.5 years ago

Dual indexes.........

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