I've been doing some alignments of a bunch of proteins using ClustalW. So I have a bunch of alignments in the format of
WP_069014636.1 -------------------FYGNLSD-SLLQKHKDDAR--------EVSQIGMGVIRYFY
WP_162272499.1 -------------------FYGNLSR-SLLQKHKDDAR--------EVSQIGMGVIRYFY
Only difference is I have around 130 proteins and they are full length proteins instead of just this small section. (I just consolidated everything for the sake of space) How can I get a distance score for each protein to one another? Is there any good tools out there for figuring this out? I've been looking and it seems the Geneious can do this, but you need the license to do so. Any recommendations?