Demultiplexing/ separating multiple hashtags in multi-modal sc-seq

Question

[harithaa]

Hello everyone :)

I need help from Single-Cell multi modal enthusiasts! We have performed 10x 5' seq -- TCR seq, CITE-seq and gene expression of PBMCs.

To get a closer look a CD4 enriched fraction of the PBMCs was also taken and same multi modal seq was done.

And each patient has two time points.

So now, I have 4 hashtags (oligonucleotides) for the 2 diff time points and for two levels (whole PBMCs and enriched fraction) apart from all the hashtags for the CITE-seq .

Essentially what I have now is only one resultant matrix file for each person at each modality (or 2 fastq (r1 and r2) for gene exp and so on).

How do I get back the information on which time point and which level of enrichment things belong to?

[fouerghi20]

Did you ever figure out how to quantify the HTOs?