Hi, I am a Ph.D student and have sequence of all chromosomes of Rice in FASTA format and I want to find more defence related and disease resistant genes (like Xa), proteins(R proteins, PRR) and QTLs (qBBR11-1). So, for this is performing functional annotation is the correct way to find? Also, for this purpose is using BLAST2 GO or WEGO Tool or GenSAS or Galaxy (Augustus)? If there are any other tools kindly suggest me.
Edit: Removed personal information.
So, these GO numbers can be retrieved from the annotation data of all the rice species from NCBI ? Also, I have to find and study more such genes, proteins and QTLs which are not mentioned before, so, in this case I have to perform the annotation ?