Biosynthetic gene clusters

Question

[amy]

Hello everyone!

I would like to ask for help/advice regarding my methods. I have a metagenome data that I have assembled using metaspades, binned using metabat2, and annotated using Prokka. I only extracted specific genera of bacteria from the metadata. I wanted to analyze the biosynthetic gene clusters of these MAGs. I used anti-smash and Bagel4 for this but my question is.. How can I tell if a BGC have antiviral potential without the need of in vitro analysis?

The results I got from anti-smash looks like this (attached photo). Thank you very much! I hope you can help me.

[andres.firrincieli]

How can I tell if a BGC have antiviral potential without the need of in vitro analysis?

You can tell only if the product of reference cluster: Most similar known cluster has known antiviral activity

[amy]

Thank you for your reply, sir.

How about the BGCs without hits to Most similar known cluster?

[andres.firrincieli]

How about the BGCs without hits to Most similar known cluster?

The only thing you can do is a literature search using the Type name: i.e. "lasso peptide" AND "antiviral".

[amy]

Thank you so much, sir!