Entering edit mode
3.9 years ago
Kevin
▴
70
I ran a feature barcode analysis using cellranger count following the steps from illumina website found here.
For the most part the analysis turned out well and the results had no errors or warnings. However, there was a set of 4 features in my feature reference csv and only 3 of them are included in the cellranger count analysis tab.
The 4 features are BM_1, BM_2, BM_3, and BM_4. BM_3 is missing from the cellranger count analysis tab but I have no idea when it may have dropped out or how to find out. The others are all present so I don't think there was an issue with the format in the csv or anything like that.
Features:
BM_1, SN28, R2, 5PNNNNNNNNNN(BC)NNNNNNNNN, ATCGACGATCGACTG, Antibody Capture
BM_2, SN41, R2, 5PNNNNNNNNNN(BC)NNNNNNNNN, GCTAGCTAGCTAGCT, Antibody Capture
BM_3, SP28, R2, 5PNNNNNNNNNN(BC)NNNNNNNNN, GACCATACGATTCAG, Antibody Capture
BM_4, SP41, R2, 5PNNNNNNNNNN(BC)NNNNNNNNN, ATAGTCGACTGACTG, Antibody Capture
