Quantifying expression levels of IsoSeq reads with Kallisto

Question

[rubic]

Hi, I have IsoSeq read data and I would like to quantify expression levels of novel isoforms (which I integrate to the reference genome) using Kallisto. Any idea of specific Kallisto usage for this case?

Answer 1

[dsull]

This is long-read sequencing data. Currently, most standard tools (including kallisto) are only suitable for short read sequencing data.