Entering edit mode
3.4 years ago
jinghuang800
▴
10
Hi everyone, I'm beginning in bioinformatics and I'm working on a seurat project now.
I noticed that in Seurat tutorial, they use subset = nFeature_RNA > 200 & nFeature_RNA < 2500 & percent.mt < 5 for filter.
The value of data in my project seems much larger than in the tutorial. The VlnPlot and FeatureScatter from my data showed below. How can I choose filter parameters for nFeature and percent.mt? Can I decide the parameter based on these two plots? Are there other ways to do the decision?
The cell is ec cell.
