Entering edit mode
2.0 years ago
dew
▴
10
Dear all,
Do you know how to generate the results to all genes(-t gene already used)?
featureCounts -T 10 -s 2 -p -t gene -a gencode.v40.primary_assembly.annotation.gtf -o gene_count.txt -g gene_name *bam
Thanks a lot!
So should the pseudogenes be used in the downstream DEGs finding? Thanks! Best!
No problem :).
I am afraid that I can't answer to that question. It depends on your hypothesis and experiment. Are you interested in the expression patterns of pseudogenes? Having differentially expressed pseudogenes is something that you are looking for in your experimental design?
Thanks! Got it. Thank you very much!Best!